A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein
A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein
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具有广泛反应性的单克隆抗体可检测乙型肝炎病毒 X 蛋白的多种基因型
DOI：
10.1007/s00705-014-2111-6
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发表时间：
2014-10-01
影响因子：
2.7
通讯作者：
Liu, Jing
中科院分区：
文献类型：
--
作者：
Wei, Lili;Shen, Zhongliang;Liu, Jing
研制了一种高特异性和广谱反应性的抗乙肝病毒X蛋白(HBX)蛋白的单抗，通过免疫印迹和免疫荧光检测了在培养细胞中过度表达的8种已知HBV型中的7种HBX蛋白。用该单抗对培养的肝细胞HBx表达水平的评价表明，瞬时稳定转染HBx基因的细胞表达的HBx蛋白远低于瞬时转染法研究HBx功能的HBx过表达的细胞。这种敏感且具有广泛反应性的抗HBx单抗的问世，将使对HBx功能的更多定量研究成为可能。
A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions.