Unique Insights in the Cervicovaginal Lactobacillus iners and L. crispatus Proteomes and Their Associations with Microbiota Dysbiosis.
Unique Insights in the Cervicovaginal Lactobacillus iners and L. crispatus Proteomes and Their Associations with Microbiota Dysbiosis.
复制标题
DOI：
10.1371/journal.pone.0150767
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
van de Wijgert JH
中科院分区：
文献类型：
--
作者：
Borgdorff H;Armstrong SD;Tytgat HL;Xia D;Ndayisaba GF;Wastling JM;van de Wijgert JH
以乳酸杆菌为主的宫颈阴道微生物群（VMB）保护妇女免受不良生殖健康结果的影响，但乳酸杆菌的作用。VMB中的iners知之甚少。我们的目的是探讨宫颈阴道L。iners和L.卷曲蛋白质组和VMB组成。阴道蛋白质组的50名卢旺达妇女在高艾滋病毒风险，分为四个VMB组（基于16 S rDNA微阵列结果），进行了研究，通过质谱使用宫颈阴道灌洗（CVL）样品。仅L的16 S结果为阳性的样本。iners和/或L.在随后的比较蛋白质分析中包括每组内的卷曲乳杆菌：卷曲乳杆菌主导的VMB簇（具有16 S-证明的L. iners（ni）= 0，并且用16 S证明了L. crispatus（nc）= 5），L. iners主导的VMB集群（ni = 11，nc = 4），中度生态失调（ni = 12，nc = 2）和重度生态失调（ni = 8，nc = 2）。对L. iners和L.比较了VMB组间卷曲肌的变化。鉴定了40种乳杆菌属蛋白，其中7种为乳杆菌属特异性蛋白。iners和L.卷曲的L.在患有L.与患有L. iners主导的VMB，不依赖于阴道pH和L。不多。此外，L.葡萄糖酸脱氢酶（DPS）、葡萄糖磷酸脱氢酶（GAPDH）、葡萄糖醛酸糖苷酶（GPI）和果糖二磷酸醛缩酶（ALDO）与阴道pH值呈显著负相关。crispatus显示出与生态失调相关的类似的负的但不显著的趋势。大多数鉴定的乳杆菌蛋白具有保守的细胞内功能，但它们在CVL上清液中的高丰度可能意味着额外的细胞外（兼职）作用。我们的研究结果表明，这些蛋白质在维持乳杆菌占主导地位的VMB中可能是重要的。需要进行功能研究，以调查它们在阴道细菌群落中的作用，以及它们是否可以用于预防阴道生态失调。
A Lactobacillus-dominated cervicovaginal microbiota (VMB) protects women from adverse reproductive health outcomes, but the role of L. iners in the VMB is poorly understood. Our aim was to explore the association between the cervicovaginal L. iners and L. crispatus proteomes and VMB composition. The vaginal proteomes of 50 Rwandan women at high HIV risk, grouped into four VMB groups (based on 16S rDNA microarray results), were investigated by mass spectrometry using cervicovaginal lavage (CVL) samples. Only samples with positive 16S results for L. iners and/or L. crispatus within each group were included in subsequent comparative protein analyses: Lactobacillus crispatus-dominated VMB cluster (with 16S-proven L. iners (ni) = 0, and with 16S-proven L. crispatus (nc) = 5), L. iners-dominated VMB cluster (ni = 11, nc = 4), moderate dysbiosis (ni = 12, nc = 2); and severe dysbiosis (ni = 8, nc = 2). The relative abundances of proteins that were considered specific for L. iners and L. crispatus were compared among VMB groups. Forty Lactobacillus proteins were identified of which 7 were specific for L. iners and 11 for L. crispatus. The relative abundances of L. iners DNA starvation/stationary phase protection protein (DPS), and the glycolysis enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate isomerase (GPI), were significantly decreased in women with L. iners-containing dysbiosis compared to women with a L. iners-dominated VMB, independent of vaginal pH and L. iners abundance. Furthermore, L. iners DPS, GAPDH, GPI, and fructose-bisphosphate aldolase (ALDO) were significantly negatively associated with vaginal pH. Glycolysis enzymes of L. crispatus showed a similar negative, but nonsignificant, trend related to dysbiosis. Most identified Lactobacillus proteins had conserved intracellular functions, but their high abundance in CVL supernatant might imply an additional extracellular (moonlighting) role. Our findings suggest that these proteins can be important in maintaining a Lactobacillus-dominated VMB. Functional studies are needed to investigate their roles in vaginal bacterial communities and whether they can be used to prevent vaginal dysbiosis.