Neural differentiation potential of peripheral blood- and bone-marrow-derived precursor cells
Neural differentiation potential of peripheral blood- and bone-marrow-derived precursor cells
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DOI：
10.1016/j.brainres.2006.09.044
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发表时间：
2006-12-06
期刊：
影响因子：
2.9
通讯作者：
Kocsis, Jeffery D.
中科院分区：
文献类型：
--
作者：
Kim, Sangnyon;Honmou, Osamu;Kocsis, Jeffery D.
在实验动物模型中，已经报道了从成人骨髓（BMSCs）制备的间充质干细胞（MSCs）的移植可以改善几种CNS疾病的功能缺陷。通过选择在适当的培养条件下生长至汇合的塑料贴壁细胞作为扁平成纤维细胞样细胞来富集骨髓中的MSC。尽管外周血中的干细胞/前体细胞被广泛用于造血系统的重建，但外周血来源的塑料粘附前体/干细胞（PMSC）是否可以分化为神经谱系尚不完全清楚。为了比较PMSCs和BMSCs体外神经分化的潜力，从成年大鼠制备BMSCs和PMSCs并在培养中扩增。虽然PMSCs的生长速度低于BMSCs，但免疫细胞化学和RT-PCR分析表明，在EGF和bFGF的存在下，这两种MSC类型均被成功诱导为巢蛋白阳性神经球。在撤除有丝分裂原后，这些细胞可分化为神经元或胶质纤维酸性蛋白阳性的神经元。因此，我们的研究结果表明，潜在的使用PMSCs的细胞治疗中枢神经系统疾病。(c)2006 Elsevier B. V.保留所有权利。
Transplantation of mesenchymal stem cells (MSCs) prepared from adult bone marrow (BMSCs) has been reported to ameliorate functional deficits in several CNS diseases in experimental animal models. Bone marrow was enriched in MSCs by selecting for plastic-adherent cells that were grown to confluency in appropriate culture conditions as flattened fibroblast-like cells. Despite the fact that the stem/precursor cells in peripheral blood are widely used for reconstruction in the hematopoietic system, it is not fully understood whether peripheral blood-derived plastic-adherent precursor/stem cells (PMSCs) can differentiate into a neural lineage. To compare the potential of PMSCs and BMSCs for neural differentiation in vitro, BMSCs and PMSCs were prepared from the adult rat and expanded in culture. Although the growth rate of PMSCs was less than BMSCs, immunocytochemical and RT-PCR analyses indicated that both MSC types were successfully induced to nestin-positive neurospheres in the presence of EGF and bFGF. After withdrawal of the mitogens, these cells could differentiate into neurofilament-positive neurons or GFAP-positive glia. Thus, our findings suggest the potential use of PMSCs for a cell therapy in CNS diseases. (c) 2006 Elsevier B.V. All rights reserved.