Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts
Molecular markers distinguish bone marrow mesenchymal stem cells from fibroblasts
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DOI：
10.1016/j.bbrc.2005.04.118
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发表时间：
2005-06-24
影响因子：
3.1
通讯作者：
Kato, Y
中科院分区：
文献类型：
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作者：
Ishii, M;Koike, C;Kato, Y
为了鉴定间充质干细胞（MSC），我们用RT-PCR和真实的时间PCR比较了人骨髓MSC（11株）和人成纤维细胞（4株）的基因表达谱。MHC-DR-α、MHC-DR-β、MHC-DR相关蛋白CD 74的信使RNA水平。组织因子途径抑制物-2和neuroserpin在MSC中比在成纤维细胞中高得多，即使存在大的个体间差异。MSC中肾上腺髓质素、载脂蛋白D、C型凝集素超家族成员-2、胶原XV α 1、CUG三联体重复RNA结合蛋白、基质金属蛋白酶-1、蛋白酪氨酸激酶-7和Sam 68样磷酸酪氨酸蛋白/T-STAR的表达低于成纤维细胞。流式细胞仪分析表明，细胞表面MHC-DR的表达也高于在成纤维细胞中的MSC。成骨分化后MHC-DR表达下降，而肾上腺髓质素-成骨细胞活性的有力刺激物-随着胶原蛋白XV α 1和载脂蛋白D的表达成骨分化后增加。在这项研究中确定的标记基因应该是有用的MSC在基础和临床研究的特点。(c)2005年爱思唯尔公司All rights reserved.
To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-alpha, MHC-DR-beta, MHC-DR-associated protein CD74. tissue factor pathway inhibitor-2, and neuroserpin were much higher in MSC than in fibroblasts, even in the presence of large interindividual variations. Those of adrenomedullin, apolipoprotein D, C-type lectin superfamily member-2, collagen type XV alpha 1, CUG triplet repeat RNA-binding protein, matrix metalloproteinase-1, protein tyrosine kinase-7, and Sam68-like phosphotyrosine protein/T-STAR were lower in MSC than in fibroblasts. FACS analysis showed that cell surface expression of MHC-DR was also higher in MSC than in fibroblasts. MHC-DR expression decreased after osteogenic differentiation, whereas the expression of adrenomedullin-a potent stimulator of osteoblast activity-along with collagen XV alpha 1 and apolipoprotein D increased after osteogenic differentiation. The marker genes identified in this Study should be useful for characterization of MSC both in basic and clinical studies. (c) 2005 Elsevier Inc. All rights reserved.