Bioactive molecule optimized for biofilm reduction related to childhood caries
Bioactive molecule optimized for biofilm reduction related to childhood caries
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DOI：
10.2217/fmb-2019-0144
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发表时间：
2019-09-01
影响因子：
3.1
通讯作者：
Sardi, Janaina C. O.
中科院分区：
文献类型：
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作者：
Bombarda, Gabriela F.;Rosalen, Pedro L.;Sardi, Janaina C. O.
目的评价新型硝基查尔酮(NC-E08)对白色念珠菌和变形链球菌的抗菌活性及其毒性。材料与方法：测定其对白色念珠菌和变形链球菌的最低抑菌浓度(MIC)和最低杀菌浓度/最低杀菌浓度(MFC)，以及对人牙龈成纤维细胞和梅隆格氏杆菌的抗菌膜潜力和毒性。对梅氏革兰氏菌进行感染治疗。结果与结论：NC-E08对白色念珠菌MIC值为0.054 mM，对变形链球菌的MIC值为0.013 mM，10倍MIC处理后，两株菌的生物被膜均减少4.0log10，白色念珠菌和变形链球菌混合生物膜的存活率分别为6.0log10和4.0log10。NC-E08对人牙龈成纤维细胞和梅氏革兰氏菌无细胞毒性。NC-E08幼虫感染后保护率达90%(p
To evaluate antimicrobial activity of a new nitrochalcone (NC-E08) against Candida albicans and Streptococcus mutans, and its toxicity. Materials & methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration/minimum fungicidal concentration (MFC) were determined against C. albicans and S. mutans, as well as antibiofilm potential and toxicity (human gingival fibroblast and Galleria mellonella). Infection and treatment were performed in G. mellonella. Results & conclusion: NC-E08 showed antimicrobial activity in C. albicans (MIC: 0.054 mM) and S. mutans (MIC: 0.013 mM); 10xMIC treatment reduced 4.0 log10 biofilms for both strains and there was a reduction in survival of mixed biofilms of C. albicans and S. mutans (6.0 and 4.0 log10, respectively). NC-E08 showed no cytotoxicity in human gingival fibroblast cells and G. mellonella. NC-E08 after larval infection protected them 90% (p