Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor
Selective monitoring of vascular cell senescence via β-Galactosidase detection with a fluorescent chemosensor
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DOI：
10.1016/j.snb.2018.07.171
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Eun-Joong Kim;Arup Podder;Mrinmoy Maiti;Jong min Lee;B. Chung;S. Bhuniya
中科院分区：
文献类型：
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作者：
Eun-Joong Kim;Arup Podder;Mrinmoy Maiti;Jong min Lee;B. Chung;S. Bhuniya
设计并合成了一种新的衰老响应荧光探针（SRP），用于检测衰老细胞中β-半乳糖苷酶的活性。在β-半乳糖苷酶的存在下，t probeSRPat 495 nm的紫外吸收增加。与β-半乳糖苷酶（0.1 U/mL）孵育后，其在λ em 545 nm处的荧光增强了27倍。SRP探针对β-半乳糖苷酶无毒性，具有高度的化学选择性。高的细胞活力和化学选择性的probeSRP提供了它是一个合适的标记，用于评估活细胞中的细胞衰老。使用这种探针，H2 O2诱导的细胞衰老的人脐静脉细胞（HUVECs）可以区分从正常细胞的荧光标记的细胞的程度的基础上。此外，它优先定位于酸性溶酶体。总之，SRP是一种独特的化学传感器，可以提供血管内皮细胞衰老的临床前信息。
A new senescence-responsive fluorescent probe (SRP) was developed for the detection of β-galactosidase activity in senescent cells. UV-absorption of t probeSRPat 495 nm was increased in the presence of β-galactosidase. Its fluorescence at λem545 nm increased ∼27-fold upon incubation with β-galactosidase (0.1 U/mL). TheSRPprobe is non-toxic and highly chemoselective for β-galactosidase. The high cell viability and chemoselcivity of probeSRPoffer it to be a suitable marker for assessing cell senescence in live cells. Using this probe, H2O2-induced cellular senescence of human umbilical vein cells (HUVECs) could be distinguished from normal cells based on the extent of fluorescent labeling of the cells. Moreover, it was preferentially localized in acidic lysosomes. Overall,SRPis a unique chemosensor that can provide preclinical information on cell senescence in vascular endothelial cells.