Identification of pigment cell antigens defined by vitiligo antibodies.
Identification of pigment cell antigens defined by vitiligo antibodies.
复制标题
白癜风抗体定义的色素细胞抗原的鉴定。
DOI：
10.1111/1523-1747.ep12555773
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Bystryn,JC
中科院分区：
文献类型：
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作者：
Cui,J;Harning,R;Henn,M;Bystryn,JC
白癜风患者有循环抗体的色素细胞。为了进一步表征这种反应并鉴定白癜风抗体所定义的抗原，通过免疫沉淀和SDS-PAGE分析色素细胞和对照细胞上125I标记的细胞抗原，对23例白癜风患者和22例不相关疾病患者的血清进行了分析。色素细胞抗原抗体在白癜风患者中有18例（78%），而在对照组中只有3例（14%）（p <0.05）。抗体针对一种或多种分子量（MW）（以千道尔顿（kD）计）为约35、40 - 45、75、90或150的抗原。最常见的反应针对40 - 45-kD、75-kD和90-kD抗原。这些抗原的抗体存在于74%，57%和35%的白癜风患者与14%，9%和0%的对照个体。35-kD和90-kD抗原优先在人色素细胞上表达，而40 - 45-，75-和150-kD抗原在色素和对照细胞上表达。这些抗原标记的乳过氧化物酶技术，表明它们是细胞表面抗原。这些结果证实色素细胞抗体与白癜风有关。这些抗体针对几种细胞表面抗原，其中一些优先在色素细胞上表达。
Patients with vitiligo have circulating antibodies to pigment cells. To characterize this response further and to identify the antigens defined by vitiligo antibodies, sera of 23 patients with vitiligo and 22 patients with unrelated conditions were analyzed by immunoprecipitation and SDS-PAGE analysis of125I-labeled cell antigens on pigment and control cells. Antibodies to pigment cell antigens were present in 18 (78%) of the patients with vitiligo but in only three (14%) of the control patients (p < 0.05). The antibodies were directed to one or more antigens with molecular weight (MW) in kilo-daltons (kD) of approximately 35, 40–45, 75, 90, or 150. The responses were most commonly directed to the 40–45-kD, 75-kD, and 90-kD antigens. Antibodies to these antigens were present in 74%, 57%, and 35% of vitiligo patients versus in 14%, 9%, and 0% of control individuals. The 35-kD and 90-kD antigens were preferentially expressed on human pigment cells, whereas the 40–45-, 75-, and 150-kD antigens were expressed on both pigment and control cells. These antigens were labeled by the lactoperoxidase technique, suggesting that they are cell surface antigens. These results confirm that antibodies to pigment cells are associated with vitiligo. These antibodies are directed to several cell surface antigens, some of which are preferentially expressed on pigment cells.