Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
复制标题
生物功能镁涂层 Ti6Al4V 支架可在体外和体内增强骨生成和血管生成，用于骨科应用
DOI：
10.1016/j.bioactmat.2020.04.019
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发表时间：
2020-09-01
影响因子：
18.9
通讯作者：
Guo, Zheng
中科院分区：
文献类型：
--
作者：
Gao, Peng;Fan, Bo;Guo, Zheng
多孔钛基支架成骨和骨整合不足限制了其进一步的应用。早期血管生成对于支架存活是重要的。因此，有必要在钛支架上开发具有成骨和血管生成特性的多功能表面。在这项研究中，生物功能镁涂层沉积在多孔Ti6 Al 4V支架。对于骨整合和成骨分析，体外研究表明，与裸Ti6 Al 4V共培养相比，镁涂层Ti6 Al 4V与MC 3 T3-E1细胞共培养可改善细胞增殖、粘附、细胞外基质（ECM）矿化和ALP活性。此外，与镁涂层Ti6 Al 4V一起培养的MC 3 T3-E1细胞显示出显著更高的成骨相关基因表达。体内研究包括荧光染料标记、显微计算机断层扫描和镁涂层Ti6 Al 4V支架的组织学检查，表明植入后兔体内新骨再生显著增加。对于血管生成研究，镁涂层的Ti6 Al 4V改善HUVECs的增殖、粘附、管形成、伤口愈合和Transwell能力。用镁涂层Ti6 Al 4V培养的HUVEC显示出显著更高的血管生成相关基因（HIF-1 α和VEGF）表达。微血管造影分析显示，镁涂层Ti6 Al 4V支架可显著促进血管形成。本研究扩大了镁的应用范围，并为传统的多孔Ti6 Al 4V支架提供了一种可选的选择，具有增强的成骨和血管生成，以进一步骨科应用。
The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.