Rayleigh and Rayleigh-Debye-Gans light scattering intensities and spetroturbidimetry of dispersions of unilamellar vesicles and multilamellar liposomes
Rayleigh and Rayleigh-Debye-Gans light scattering intensities and spetroturbidimetry of dispersions of unilamellar vesicles and multilamellar liposomes
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DOI：
10.1016/j.jcis.2020.05.085
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发表时间：
2020-10-15
影响因子：
9.9
通讯作者：
Franses, Elias, I
中科院分区：
文献类型：
--
作者：
Hsieh, An-Hsuan;Corti, David S.;Franses, Elias, I
假设：由于在囊泡和脂质体中，厚度为d(b)的表面活性剂双层的体积分数(s)小于1，因此瑞利散射强度(R)和浊度对颗粒半径a的依赖性弱于与a(3)成正比的均匀球体。Rayleigh-Debye-Gans （RDG）散射强度和浊度对a的依赖性也较弱。所做的工作：推导了有效相对折射率与a、d(b)和d(w)（水层厚度）的关系。对R和RDG散射分别解析导出了单散射和独立散射的比瑞利比R-theta**和比浊度tau**。对阳离子双链表面活性剂二十二烷基二甲基溴化铵（DDAB）在25℃下的浊度测定数据与浊度预测结果进行了比较。结果：对于R散射，对于囊泡，R-theta**和tau**与a(2)d(b)成正比，对于脂质体，R (3) (d(b/)d（w)+d(b)）成正比。对于RDG和颗粒半径20- 1000nm， tau**与a(n)成正比，其中囊泡的n为2 ~ 0.4，脂质体的n为2 ~ 1.1。DDAB囊泡的浊度数据与RDG预测一致，RDG也用于估计囊泡的大小。RDG适用于< 800 nm的脂质体和更大尺寸的囊泡。(C) 2020爱思唯尔公司版权所有。
Hypothesis: Since the volume fraction of the surfactant bilayer(s), of thickness d(b), in a vesicle and liposome is smaller than one, the dependences of the Rayleigh (R) scattering intensity and turbidity on the particle radius a are weaker than those for a homogeneous sphere, which are proportional to a(3). The dependences of the Rayleigh-Debye-Gans (RDG) scattering intensity and turbidity on a are also weaker. Work done: The dependences of the effective relative refractive index on a, d(b), and d(w) (water layer thickness) were derived. The specific Rayleigh ratio R-theta** and the specific turbidity tau** for single and independent scattering were derived analytically for R and RDG scattering. Spectroturbidimetry data at 25 degrees C for a cationic double-chain surfactant, didodecyldimethylammonium bromide (DDAB) were compared to the turbidity predictions. Findings: For R scattering, R-theta** and tau** are proportional to a(2)d(b) for vesicles, and to a(3) (d(b/)d(w)+d(b)) for liposomes. For RDG and particle radii 20-1000 nm, tau** is proportional to a(n), where n is 2 to 0.4 for vesicles and 2 to 1.1 for liposomes. Turbidity data for DDAB vesicles are consistent with the RDG predictions, which are also used to estimate the vesicles' sizes. RDG applies to liposomes < 800 nm and to much larger sizes for vesicles. (C) 2020 Elsevier Inc. All rights reserved.