Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.
Use of a single monoclonal antibody to determine the susceptibilities of herpes simplex virus type 1 and type 2 clinical isolates to acyclovir.
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使用单一单克隆抗体测定 1 型和 2 型单纯疱疹病毒临床分离株对阿昔洛韦的敏感性。
DOI：
10.1128/cdli.9.6.1379-1381.2002
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
McSharry,JamesJ
中科院分区：
文献类型：
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作者：
Chutkowski,Christine;Olson,Betty;McDonough,Ann;Mahoney,James;McSharry,JamesJ
本报告描述了一种流式细胞术药物敏感性试验，使用一个单一的荧光染料标记的单克隆抗体，以确定阿昔洛韦敏感性的单纯疱疹病毒（HSV）1型或2型临床分离株。该试验产生HSV临床分离株的50%有效剂量（使抗原阳性细胞数量减少50%的药物浓度），与空斑减少试验获得的剂量相当。
This report describes a flow cytometry drug susceptibility assay that uses a single fluorochrome-labeled monoclonal antibody to determine the acyclovir susceptibilities of herpes simplex virus (HSV) type 1 or type 2 clinical isolates. This assay yields 50% effective doses (drug concentrations that reduce the number of antigen-positive cells by 50%) for HSV clinical isolates that are equivalent to those obtained with the plaque reduction assay.