A Comparative Study on Skin and Plasma Advanced Glycation End Products and Their Associations with Arterial Stiffness
A Comparative Study on Skin and Plasma Advanced Glycation End Products and Their Associations with Arterial Stiffness
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DOI：
10.1159/000453581
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Wang, Ji-Guang
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作者：
Liu, Chang-Yuan;Huang, Qi-Fang;Wang, Ji-Guang
背景资料：我们比较了晚期糖基化终产物（AGEs）的皮肤和血浆测量值，特别关注高血压或糖尿病和前驱糖尿病患者的AGEs水平及其与疑似或诊断为高血压的门诊患者动脉僵硬度的相关性。研究方法：使用皮肤自体荧光读取器测量左前臂上的自体荧光形式的皮肤AGE积累，并以0至25范围内的任意单位表示。用酶联免疫吸附法测定血浆AGE浓度，并进行数学转换进行统计学分析。使用SphygmoCor系统（Sydney，Australia）通过颈动脉-股动脉脉搏波速度（cfPWV）评估动脉硬度。结果如下：218名参与者（96名[44.0%]男性，平均年龄51.9岁）的平均皮肤自发荧光为1.89任意单位，血浆AGE浓度为4.47 μ g/ml，cfPWV为8.0 m/s。在糖尿病或糖尿病前期患者中，皮肤自发荧光与血浆AGEs显著相关（n = 31，r = 0.37，p = 0.04），但在血糖正常的受试者中则不相关（n = 187，r = 0.05，p = 0.48）。尽管如此，两个测量值均具有显著性（p = 0.35）。在校正的多元回归分析中，血浆AGE浓度，而不是皮肤自发荧光（p >= 0.37），在所有受试者中（每增加10倍，β 0.44 m/s; p = 0.04）和男性、糖尿病和前驱糖尿病亚组中（每增加10倍，β 0.12-0.55 m/s; p = 0.04）与cfPWV显著相关。
Background: We compared skin and plasma measurements of advanced glycation end products (AGEs), with particular focus on their levels in the presence of hypertension or diabetes and prediabetes and their associations with arterial stiffness in outpatients with suspected or diagnosed hypertension. Methods: Skin AGE accumulation was measured as autofluorescence on the left forearm using the skin autofluorescence Reader and expressed in arbitrary units in the range from 0 to 25. Plasma AGE concentration was measured by the enzyme linked immunosorbent assay method and logarithmically transformed for statistical analysis. Arterial stiffness was assessed by carotid-femoral pulse wave velocity (cfPWV) using the SphygmoCor system (Sydney, Australia). Results: The 218 participants (96 [44.0%] men, mean age 51.9 years) had a mean skin autofluorescence of 1.89 arbitrary units, plasma AGE concentration of 4.47 mu g/ml, and cfPWV of 8.0 m/s. Skin autofluorescence was significantly correlated with plasma AGEs in diabetic or prediabetic patients (n = 31, r = 0.37, p = 0.04) but not in subjects with normoglycemia (n = 187, r = 0.05, p = 0.48). Nonetheless, both measurements were significantly (p = 0.35). In adjusted multiple regression analyses, plasma AGE concentration, but not skin autofluorescence (p >= 0.37), was significantly associated with cfPWV in all subjects(beta 0.44 m/s for each 10-fold increase; p = 0.04) and in subgroups of men and diabetes and prediabetes (beta 0.12-0.55 m/s for each 10-fold increase; p