The modulation of lymphocyte functions by molecules secreted by macrophages. II. Conditions leading to increased secretion
The modulation of lymphocyte functions by molecules secreted by macrophages. II. Conditions leading to increased secretion
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巨噬细胞分泌的分子对淋巴细胞功能的调节。
DOI：
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发表时间：
1976
影响因子：
15.3
通讯作者：
J. Calderón
中科院分区：
文献类型：
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作者：
E. Unanue;J. Kiely;J. Calderón
研究了富含巨噬细胞的腹膜渗出细胞培养物的淋巴刺激分子分泌。两种条件产生分泌增加：（a）向培养物中加入容易与巨噬细胞相互作用的各种试剂，如乳胶颗粒、抗体包被的红细胞、内毒素、李斯特菌或Be盐;或（B）加入活化的淋巴细胞。在第一种情况下，在摄取刺激后的第一个24或48小时内发现活性增加。在正常或蛋白胨刺激的巨噬细胞中发现活性增加，但在注射内毒素或巯基乙酸盐后的巨噬细胞中未发现。将李斯特菌感染小鼠的T淋巴细胞加入巨噬细胞培养物中，大大增加了活性。这种增加也是通过将抗原与抗原一起添加到抗原致敏的T细胞中产生的。淋巴细胞本身不分泌活性因子。在体外对胸腺细胞DNA合成和抗体形成进行了淋巴刺激活性测试。后一种测定在来自免疫小鼠的脾细胞上进行，其中一个显著的效果是刺激分化为抗体分泌细胞。在选定的培养液中观察到两种活性（胸腺细胞DNA合成和B细胞分化）的一些分离。
Cultures of peritoneal exudate cells rich in macrophages were studied for the secretion of lymphostimulatory molecules. Two conditions produced increased secretion: (a) addition to the cultures of various agents that readily interacted with macrophages, such as latex particles, antibody-coated red cells, endotoxin, Listeria organisms, or Be salt; or (b) addition of activated lymphocytes. In the first case the increased activity was found during the first 24 or 48 h after uptake of the stimuli. Increased activity was found in normal or peptone-stimulated macrophages but not in macrophages after injection of endotoxin or thioglycollate. The addition of T lymphocytes from Listeria-infected mice to macrophage cultures increased greatly the activities. This increase was also produced by addition to antigen- primed T cells together with antigen. The lymphocytes by themselves did not secrete active factors. The lymphostimulatory activities were tested on thymocyte DNA synthesis and on antibody formation in vitro. The latter assay was done on spleen cells from immunized mice where one striking effect was the stimulation of differentiation to antibody- secreting cells. Some dissociation of both activities (thymocyte DNA synthesis and B-cell differentiation) was observed with selected culture fluids.