Phosphorylation of the D1 photosystem II reaction center protein is controlled by an endogenous circadian rhythm
Phosphorylation of the D1 photosystem II reaction center protein is controlled by an endogenous circadian rhythm
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DOI：
10.1104/pp.013441
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发表时间：
2002-12-01
期刊：
影响因子：
7.4
通讯作者：
Mattoo, AK
中科院分区：
文献类型：
--
作者：
Booij-James, IS;Swegle, WM;Mattoo, AK
光依赖的D1磷酸化是高等植物所特有的，在蓝藻和藻类中是构成的。在自然日照周期的温室条件下生长的光自养高等植物少根紫草中，光系统II的磷酸化与总D1蛋白的比率(D1-P指数：[d1-P]/[d1]+[d1-P])发生了可重复的日振荡。这些振荡与光强最大的周期明显不同。温度的变化、类囊体膜上存在的D1K活性的大小、D1蛋白合成的速率或光抑制都不影响D1P值达到最大值的时间。然而，当通过将植物转移到连续光照条件来人为缩短正常日循环中的暗期时，D_1-P指数时序发生移动，并在光照后4~5h达到最大值。由此产生的昼夜振荡在连续的光线下至少持续了两个周期，这表明这种节律是内源性的(昼夜节律)，并受到外部信号的影响。
The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P index: [D1-P]/[D1] + [D1-P]) of photosystem II is shown to undergo reproducible diurnal oscillation. These oscillations were clearly out of phase with the period of maximum in light intensity. The timing of the D1-P index maximum was not affected by changes in temperature, the amount of D1 kinase activity present in the thylakoid membranes, the rate of D1 protein synthesis, or photoinhibition. However, when the dark period in a normal diurnal cycle was cut short artificially by transferring plants to continuous light conditions, the D1-P index timing shifted and reached a maximum within 4 to 5 h of light illumination. The resultant diurnal oscillation persisted for at least two cycles in continuous light, suggesting that the rhythm is endogenous (circadian) and is entrained by an external signal.