Identity elements used in export of mRNAs
Identity elements used in export of mRNAs
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DOI：
10.1016/s1097-2765(02)00454-9
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发表时间：
2002-03-01
期刊：
影响因子：
16
通讯作者：
Mattaj, IW
中科院分区：
文献类型：
--
作者：
Ohno, M;Segref, A;Mattaj, IW
不同种类的RNA通过不同的因子从细胞核输出。我们证明，U1 snRNA的出口像一个mRNA插入前mRNA内含子或正义或反义mRNA外显子序列。mRNA特异性因子被募集到拼接或延长的U1 RNA上，而U snRNA特异性因子则不然，这表明RNA中足够长度的非结构化区域充当mRNA身份的主导决定因素。输出后，剪接的U1 RNA经历细胞质成熟，但不重新输入细胞核。这些数据提供了深入了解不同类别的核RNA的歧视机制，并表明，两个相同序列的RNA可以有不同的细胞质命运取决于他们的出口模式。
Different classes of RNA are exported from the nucleus by distinct factors. We demonstrate that U1 snRNA is exported like an mRNA on insertion of a pre-mRNA intron or either sense or antisense mRNA exon sequences. mRNA-specific factors are recruited onto the spliced or elongated U1 RNA whereas U snRNA-specific factors are not, suggesting that an unstructured region of sufficient length in an RNA acts as a dominant determinant of mRNA identity. After export, spliced U1 RNA undergoes cytoplasmic maturation but is not reimported into the nucleus. These data provide insight into mechanisms for discrimination of different classes of nuclear RNA and demonstrate that two RNAs of identical sequence can have distinct cytoplasmic fates depending on their mode of export.