decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary
decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary
复制标题
DOI：
10.1016/s0092-8674(00)81424-5
复制
发表时间：
1998-07-24
期刊：
影响因子：
64.5
通讯作者：
Spradling, AC
中科院分区：
文献类型：
--
作者：
Xie, T;Spradling, AC
干细胞被认为占据了由邻近细胞建立的特殊局部环境或小生境，使它们具有自我更新的能力。果蝇卵巢中的每个卵巢管包含两个生殖干细胞，周围是一组表达hedgehog和wingless的分化体细胞。在这里，我们表明，BMP 2/4同源物decapentaplegic（DPP）是特别需要维持女性生殖系干细胞，并促进其分裂。过表达dpp阻断生殖系干细胞分化。相反，dpp或其受体（萨克斯管）的突变会加速干细胞的损失并延缓干细胞的分裂。我们构建了突变的生殖系干细胞克隆，以证明dpp信号直接被生殖系干细胞接收。因此，dpp信号有助于确定一个控制生殖系干细胞增殖的小生境。
Stem cells are thought to occupy special local environments, or niches, established by neighboring cells that give them the capability for self-renewal. Each ovariole in the Drosophila ovary contains two germline stem cells surrounded by a group of differentiated somatic cells that express hedgehog and wingless. Here we show that the BMP2/4 homolog decapentaplegic (dpp) is specifically required to maintain female germline stem cells and promote their division. Overexpression of dpp blocks germline stem cell differentiation. Conversely, mutations in dpp or its receptor (saxophone) accelerate stem cell loss and retard stem cell division. We constructed mutant germline stem cell clones to show that the dpp signal is directly received by germline stem cells. Thus, dpp signaling helps define a niche that controls germline stem cell proliferation.