Novel nanoscale topography on poly(propylene carbonate)/poly(epsilon-caprolactone) electrospun nanofibers modifies osteogenic capacity of ADCs
Novel nanoscale topography on poly(propylene carbonate)/poly(epsilon-caprolactone) electrospun nanofibers modifies osteogenic capacity of ADCs
复制标题
聚（碳酸丙烯酯）/聚（ε-己内酯）电纺纳米纤维上的新型纳米级形貌改变了 ADC 的成骨能力
DOI：
10.1039/c5ra15841a
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Guo Gang
中科院分区：
文献类型：
--
作者：
Wang YueLong;Deng JiaoJiao;Fan RangRang;Tong AiPing;Zhang XiaoNing;Zhou LiangXue;Zheng Yu;Xu JianGuo;Guo Gang
在本研究中，我们利用一种简单的工艺，将新型聚碳酸亚丙酯/聚己内酯(ε/PCL)纳米纤维电纺成具有特殊纳米级形貌的聚碳酸酯/聚己内酯纳米纤维。用傅立叶变换红外光谱(FT-IR)、X射线衍射仪(XRD)、热重分析(TG)、差示扫描量热仪(DSC)、扫描电子显微镜(SEM)、水接触角(WCA)、拉伸试验和比表面积分析(BET)对所制备的支架进行了表征。为了确定纳米形貌是否改变了间充质干细胞的黏附、增殖和分化行为，将脂肪组织来源的基质细胞(ADCs)培养在具有光滑纳米形貌的纯PPC电纺支架和具有纳米形貌的PPC/PCL(20wt%PCL)杂化支架上。结果表明，具有特殊表面连通孔的PPC/PCL电纺纤维具有较高的比表面积，并能促进ADC的黏附和增殖。根据茜素红S染色和冯-科萨染色，表面连通的支架表面有更多的钙结节沉积。
In this study, we electrospun novel poly(propylene carbonate)/poly(ε-caprolactone) (PPC/PCL) nanofibers with a special nanoscale topography using a simple process. The characteristics of the fabricated scaffolds were investigated using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analyses (XRD), thermogravimetric analyses (TG), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), water contact angle measurements (WCA), tensile tests and Brunauer–Emmett–Teller (BET) surface area analysis. To determine whether the nanoscale topography altered mesenchyme stem cell adhesion proliferation and differentiation behavior, adipose tissue-derived stromal cells (ADCs) were cultured on pure PPC electrospun scaffolds with smooth nanotopography and PPC/PCL (20 wt% PCL) hybrid scaffold with nanoscale topography. The results reveal that PPC/PCL electrospun fibers with special inter-surface-connected pores possess a high BET surface area and could promote ADCs adhesion and proliferation. According to alizarin red S staining and von-Kossa staining assays, there are more calcium nodule deposits on scaffolds with inter-surface-connected pores.