The use of oligodeoxynucleotide probes in chaotrope-based hybridization solutions.
The use of oligodeoxynucleotide probes in chaotrope-based hybridization solutions.
复制标题
在基于离液剂的杂交溶液中使用寡脱氧核苷酸探针。
DOI：
10.1093/nar/19.19.5143
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发表时间：
1991
影响因子：
14.9
通讯作者：
Chen,L
中科院分区：
文献类型：
--
作者：
VanNess,J;Chen,L
含有离液剂的杂交溶液可用于在90°C范围内调节寡脱氧核苷酸（ODN）双链体或杂交体的热稳定性（Tmor Td）。Td的调节允许杂交溶液的配制，其允许使用探针长度、探针组成、靶类型的大多数组合的环境温度杂交，并且促进方便和快速的测定形式的开发。对三氯乙酸盐、硫氰酸盐、胍盐和其它离液盐，描述了实现ODN双链体保真度和杂交产物的最佳产率所需的条件。本文叙述了不同固体载体对Tdare的影响。此外，提出了一种方法，其使用离液序列高的化合物来减少夹心测定杂交形式中由信号ODN探针产生的背景。
Hybridization solutions containing chaotropes may be used to modulate the thermal stability (Tmor Td) of oligodeoxynucleotide (ODN) duplexes or hybrids over a 90°C range. Modulation of Tdallows formulation of hybridization solutions that permit ambient temperature hybridization using most combinations of probe length, probe composition, target type, and facilitates development of convenient and rapid assay formats. The conditions required to achieve ODN duplex fidelity, and optimal yields of hybridized product, are described for trlchloroacetate, thiocyanate, guanidinium salts and other chaotropic salts. The effects of different solid supports on Tdare described. Also, a method is presented that uses chaotropic compounds to reduce background arising from signal ODN probes in a sandwich assay hybridization format.