Invasion of Salmonella enteritidis in the tissues of reproductive organs in laying Japanese quail: an immunocytochemical study.
Invasion of Salmonella enteritidis in the tissues of reproductive organs in laying Japanese quail: an immunocytochemical study.
复制标题
DOI：
10.1093/ps/82.7.1170
复制
发表时间：
2003-07
期刊：
影响因子：
4.4
通讯作者：
T. Takata;J. Liang;H. Nakano;Y. Yoshimura
中科院分区：
文献类型：
--
作者：
T. Takata;J. Liang;H. Nakano;Y. Yoshimura
本研究的目的是确定肠炎沙门氏菌(SE)接种到日本鹌鹑蛋鸡体内是否会在生殖器官组织中定植。将5×10(7)或5×10(8)SE细胞接种于产蛋规律蛋鸡体内，处理后24或48小时分别摘除卵巢、输卵管、肾、脾、肝和大肠。石蜡切片进行SE免疫组织化学染色。SE可侵袭卵巢间质、卵泡壁和卵黄层。SE免疫反应产物多见于卵泡间质和表层的成纤维样细胞和巨噬细胞样细胞。SE免疫反应产物定位于输卵管粘膜表面、粘膜上皮和输卵管各段的间质组织中。这些组织中的粘膜上皮细胞和间质细胞胞浆中含有大量细菌。肾、脾、肝和大肠组织中也有SE免疫反应。这些结果表明，进入腹膜腔的SE细菌可以入侵和定植卵巢和输卵管组织，可能与污染卵子的产生有关。
The aim of this study was to determine whether Salmonella enteritidis (SE) inoculated into the peritoneal cavity would colonize tissues of reproductive organs in Japanese quail hens. Quail hens regularly laying were intraperitoneally inoculated with 5 x 10(7) or 5 x 10(8) SE cells, and the ovary, oviduct, kidney, spleen, liver, and large intestine were excised 24 or 48 h after the treatment. Paraffin sections of these organs were immunostained for SE. Invasion of SE was found in the tissues of the ovarian stroma, the follicular wall including superficial and theca layers, and occasionally in the granulosa layer and yolk. The SE immunoreaction product frequently was found in the fibroblast-like and macrophage-like cells in the stroma and surface layer of follicles. The SE immunoreaction products were identified on the mucosal surface, in the mucosal epithelium, and in the stromal tissues in all segments of the oviduct. Many of the bacteria were contained in the cytoplasm of mucosal epithelial cells and stromal cells in those tissues. The SE immunoreactions were also found in the tissues of kidney, spleen, and liver and in the large intestine. These results suggest that SE organisms introduced into the peritoneal cavity can invade and colonize the tissues of ovary and oviduct and may be responsible in the production of contaminated eggs.