Cultivation of Plasmodium falciparum in vitro using microbiological gas sachets that generate microaerophilic conditions.
Cultivation of Plasmodium falciparum in vitro using microbiological gas sachets that generate microaerophilic conditions.
复制标题
使用产生微需氧条件的微生物气袋在体外培养恶性疟原虫。
DOI：
10.1016/s0035-9203(98)91043-4
复制
发表时间：
1998
影响因子：
2.2
通讯作者：
A. Taylor
中科院分区：
文献类型：
--
作者：
A. Taylor
对商业上用于分离和生长弯曲杆菌的微需氧气氛发生系统进行了恶性疟原虫无性血液阶段体外培养的评估。 P 的文化。恶性疟原虫菌株 3D7 在 3.5 升密封气瓶或密封模块化培养箱内的微量滴定板中于 37°C 下培养。在密封前立即将 CampyGen 气袋放入每个容器中。在 144 小时的潜伏期内，定期通过光学显微镜评估寄生虫的成熟情况；通过测定[3H]次黄嘌呤的掺入来评估寄生虫增殖。微需氧气氛发生系统产生的条件优于常用的支持P的气体混合物。恶性疟在体外生长。增殖和总寄生虫血症低于5% O2、5% CO2 和90% N2 中获得的结果，但高于1% O2、3% CO2 和96% N2 中获得的结果。结论是，使用微生物气袋适合血期疟原虫的生长。
A microaerophilic atmosphere generation system which is commercially available for the isolation and growth ofCampylobacterwas evaluated for the cultivationin vitroof the asexual blood-stage ofPlasmodium falciparum. Cultures ofP. falciparumstrain 3D7 were incubated at 37°C in microtitre plates inside either a 3.5 litre airtight gas jar, or a sealed modular incubator chamber. A CampyGen gas sachet was placed in each vessel immediately before sealing. Parasite maturation was assessed by light microscopy at regular intervals over a 144 h incubation period; parasite proliferation was assessed by determining the incorporation of [3H]hypoxanthine. The conditions generated by the microaerophilic atmosphere generation system compared favourably with the commonly used gas mixtures for supportingP. falciparumgrowthin vitro. Proliferation and total parasitaemias were less than those attained in 5% O2, 5% CO2and 90% N2, but more than in 1% O2, 3% CO2and 96% N2. It is concluded that the use of microbiological gas sachets is suitable for the growth of blood-stage malaria parasites.