Identification of Corynebacterium spp. isolated from bovine intramammary infections by matrix-assisted laser desorption ionization-time of flight mass spectrometry
Identification of Corynebacterium spp. isolated from bovine intramammary infections by matrix-assisted laser desorption ionization-time of flight mass spectrometry
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DOI：
10.1016/j.vetmic.2014.06.028
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发表时间：
2014-09-17
影响因子：
3.3
通讯作者：
dos Santos, Marcos Veiga
中科院分区：
文献类型：
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作者：
Goncalves, Juliano Leone;Tomazi, Tiago;dos Santos, Marcos Veiga
棒状杆菌属 (spp.) 是与奶牛亚临床乳腺炎相关的最常见的病原体之一。然而，目前还没有简单、快速和可靠的方法来鉴定棒状杆菌属的物种。本研究旨在评估基质辅助激光解吸电离/质谱 (MALDI-TOF MS) 在鉴定棒状杆菌属中的有用性。从奶牛的乳腺中分离出来。棒状杆菌属通过微生物培养从牛奶样品中分离出来 (n = 180)，并通过 MALDI-TOF MS 和 16S rRNA 基因测序进行分析。使用 MALDI-TOF MS 方法，161 棒状杆菌属。在种水平上正确鉴定了 12 个分离株（89.4%），在属水平上正确鉴定了 12 个分离株（6.7%）。通过 16 S rRNA 基因测序在物种水平鉴定的大多数分离株被鉴定为牛棒状杆菌（n = 156；86.7%），也通过 MALDI-TOF MS 鉴定为牛棒状杆菌。五种棒状杆菌属使用 MALDI-TOF MS 在种水平上未正确鉴定出 2 个分离株 (2.8%)，并且认为有 2 个分离株 (1.1%) 未鉴定，因为尽管 MALDI-TOF MS 得分 >2，但仅正确鉴定了属水平。因此，MALDI-TOF MS可以作为棒状杆菌引起的牛乳房内感染的种属水平诊断的替代方法。 (C) 2014 Elsevier B.V. 保留所有权利。
Corynebacterium species (spp.) are among the most frequently isolated pathogens associated with subclinical mastitis in dairy cows. However, simple, fast, and reliable methods for the identification of species of the genus Corynebacterium are not currently available. This study aimed to evaluate the usefulness of matrix-assisted laser desorption ionization/mass spectrometry (MALDI-TOF MS) for identifying Corynebacterium spp. isolated from the mammary glands of dairy cows. Corynebacterium spp. were isolated from milk samples via microbiological culture (n = 180) and were analyzed by MALDI-TOF MS and 16S rRNA gene sequencing. Using MALDI-TOF MS methodology, 161 Corynebacterium spp. isolates (89.4%) were correctly identified at the species level, whereas 12 isolates ( 6.7%) were identified at the genus level. Most isolates that were identified at the species level with 16 S rRNA gene sequencing were identified as Corynebacterium bovis (n = 156; 86.7%) were also identified as C. bovis with MALDI-TOF MS. Five Corynebacterium spp. isolates (2.8%) were not correctly identified at the species level with MALDI-TOF MS and 2 isolates (1.1%) were considered unidentified because despite having MALDI-TOF MS scores >2, only the genus level was correctly identified. Therefore, MALDI-TOF MS could serve as an alternative method for species-level diagnoses of bovine intramammary infections caused by Corynebacterium spp. (C) 2014 Elsevier B.V. All rights reserved.