Host-Microbiota Interactions in the Pathogenesis of Antibiotic-Associated Diseases.
Host-Microbiota Interactions in the Pathogenesis of Antibiotic-Associated Diseases.
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DOI：
10.1016/j.celrep.2016.01.009
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发表时间：
2016-02-09
期刊：
影响因子：
8.8
通讯作者：
Elias JE
中科院分区：
文献类型：
--
作者：
Lichtman JS;Ferreyra JA;Ng KM;Smits SA;Sonnenburg JL;Elias JE
对宿主和微生物之间相互作用的进一步了解将为疾病的诊断、治疗和预防开辟新的途径。在这里，我们提供了一个高分辨率的视图，宿主和肠道菌群之间的动态在寄生虫诱导的肠道菌群消耗，机会性鼠伤寒沙门氏菌和艰难梭菌发病机制，并从这些扰动状态在小鼠模型中恢复。以宿主为中心的蛋白质组和微生物群落概况提供了一个细致入微的纵向视图，揭示了宿主和微生物群在不断发展的生态失调中的相互依赖关系。时间和条件特异性的分子和微生物特征是明显的，并与病原体无关的炎症指纹明确区分。我们的数据显示，从抗生素治疗或C。尽管微生物群的组成正常化，但艰难梭菌感染保留了炎症的挥之不去的特征，并且宿主反应可以通过粪便移植快速且持久地缓解。这些实验证明了这些正交的，非靶向的方法结合到胃肠道生态系统中所产生的见解。
Improved understanding of the interplay between host and microbes stands to illuminate new avenues for disease diagnosis, treatment, and prevention. Here, we provide a high-resolution view of the dynamics between host and gut microbiota during antibiotic-induced intestinal microbiota depletion, opportunistic Salmonella typhimurium and Clostridium difficile pathogenesis, and recovery from these perturbed states in a mouse model. Hostcentric proteome and microbial community profiles provide a nuanced longitudinal view, revealing the interdependence between host and microbiota in evolving dysbioses. Time- and condition-specific molecular and microbial signatures are evident and clearly distinguished from pathogen-independent inflammatory fingerprints. Our data reveal that mice recovering from antibiotic treatment or C. difficile infection retain lingering signatures of inflammation, despite compositional normalization of the microbiota, and host responses could be rapidly and durably relieved through fecal transplant. These experiments demonstrate insights that emerge from the combination of these orthogonal, untargeted approaches to the gastrointestinal ecosystem.