Increased expression of the amyloid precursor β-secretase in Alzheimer's disease
Increased expression of the amyloid precursor β-secretase in Alzheimer's disease
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DOI：
10.1002/ana.10208
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发表时间：
2002-06-01
影响因子：
11.2
通讯作者：
Evin, G
中科院分区：
文献类型：
--
作者：
Holsinger, RMD;McLean, CA;Evin, G
β-分泌酶裂解是产生 Abeta 多肽的第一步，并启动淀粉样蛋白级联反应，导致阿尔茨海默病酪蛋白酶中的神经变性。通过比较蛋白质印迹分析，我们发现，与年龄匹配的对照组相比，阿尔茨海默病患者大脑皮层中 β-分泌酶 BACE 的蛋白质表达增加了 2.7 倍。同样，我们发现，在阿尔茨海默病皮质中，β-分泌酶产生的淀粉样蛋白前体蛋白 C 末端片段的水平增加了近两倍。
beta-Secretase cleavage represents the first step in the generation of Abeta polypeptides and initiates the amyloid cascade that leads to neurodegeneration in Alzheimer's casease. By comparative Western blot analysis, we show a 2.7-fold increase in protein expression of the beta-secretase enzyme BACE in the brain cortex of Alzheimer's disease patients as compared to age-matched controls. Similarly, we found the levels of the amyloid precursor protein C-terminal fragment produced by beta-secretase to be increased by nearly twofold in Alzheimer's disease cortex.