Analysis of transcriptional response in zebrafish eleutheroembryos exposed to climbazole: Signaling pathways and potential biomarkers
Analysis of transcriptional response in zebrafish eleutheroembryos exposed to climbazole: Signaling pathways and potential biomarkers
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DOI：
10.1002/etc.4355
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发表时间：
2019-02
影响因子：
4.1
通讯作者：
Hui Zhang;Zhifeng Chen;Zenghua Qi;Shi-chao Yan;Wen-Wen Wei-Wen;Guoguang Liu;Z. Cai
中科院分区：
文献类型：
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作者：
Hui Zhang;Zhifeng Chen;Zenghua Qi;Shi-chao Yan;Wen-Wen Wei-Wen;Guoguang Liu;Z. Cai
克林巴唑是一种用于个人护理产品的抗真菌活性成分。使用后，该化学品进入水生环境，可能对鱼类造成风险。在本研究中，我们测量了暴露于环境相关的甘巴巴的斑马鱼刺五加胚胎中与多种信号通路相关的必需基因的转录效应，并预测了最坏情况的环境浓度，并通过偏最小二乘判别分析探索了潜在的生物标志物。涵盖多达 73 个基因的转录分析显示，在暴露于环境相关浓度的甘巴巴之后，斑马鱼胚胎和幼虫中昼夜节律和类固醇生成相关基因显着下调。这种外用抗真菌剂还在预测的最坏情况环境浓度下调节与炎症、氧化应激、卵母细胞成熟和性分化有关的基因转录。此外，mprα、igf3、nr1d1、nr1d2b、cyp19a1a、vtg1、il-1β 和 il-8 被选为胚胎斑马鱼接触甘巴巴后的潜在生物标志物。这些发现可以帮助我们了解斑马鱼生命早期阶段对甘巴巴的显着转录反应。未来的研究应该阐明转录调节是否转化为与相应信号通路相关的代谢表型。环境毒理学化学2019；38：794–805。 © 2019 塞塔克
Climbazole is an antifungal active ingredient used in personal care products. After application this chemical reaches the aquatic environment and may pose a risk to fish. In the present study, we measured the transcriptional effects of essential genes related to a wide range of signaling pathways on zebrafish eleutheroembryos exposed to climbazole at environmentally relevant and predicted worst‐case environmental concentrations, and explored the potential biomarkers via partial least squares discriminant analysis. Transcription analysis covering up to 73 genes revealed significant down‐regulation of circadian rhythm‐ and steroidogenesis‐related genes in zebrafish embryos and larvae after exposure to environmentally relevant concentrations of climbazole. This topical antifungal agent also modulated the transcripts of genes involved in inflammation, oxidative stress, oocyte maturation, and sexual differentiation at predicted worst‐case environmental concentrations. In addition, mprα, igf3, nr1d1, nr1d2b, cyp19a1a, vtg1, il‐1β, and il‐8 were chosen as potential biomarkers in embryonic zebrafish following exposure to climbazole. These findings can help us understand the remarkable transcriptional response to climbazole in the early life stage of zebrafish. Future research should elucidate whether the transcriptional modulation translates into metabolic phenotypes associated with the corresponding signaling pathways. Environ Toxicol Chem 2019;38:794–805. © 2019 SETAC