A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis
A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis
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DOI：
10.1099/mic.0.29158-0
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发表时间：
2006-10-01
期刊：
影响因子：
2.8
通讯作者：
Kjelleberg, Staffan
中科院分区：
文献类型：
--
作者：
Dalisay, Doralyn S.;Webb, Jeremy S.;Kjelleberg, Staffan
这项研究表明，附着的海洋细菌Pseudoalteromonastunicata的含纤维素的表面的绿色石莼australis介导的甘露糖敏感的血凝素（MSHA样）菌毛。我们已经确定了一个MSHA菌毛生物发生基因位点，命名为msh/1/2 JKLMNEGFBACDOPQ，它显示出显着的同源性，就其遗传特征和组织，霍乱弧菌的MSHA菌毛生物发生基因位点。电子显微镜研究表明，野生型披衣侧耳细胞表达柔性皮利，排列在细胞表面。在mshJ区域中具有转座子插入的被衣假单胞菌突变体（SM 5）显示非纤毛表型。使用SM 5，已经证明MSHA菌毛促进被囊泡囊藻野生型细胞在聚苯乙烯微量滴定板中的附着，以及对微晶纤维素和U.澳大利亚。被衣假单胞菌也表现出增加的菌毛生产响应纤维素及其单体成分纤维二糖。因此，MSHA菌毛的功能作为一个决定因素的附着在披衣藻，它提出的理解表面传感机制显示披衣藻将提供洞察特定的生态相互作用，发生在这种细菌和高等海洋生物之间。
This study demonstrates that attachment of the marine bacterium Pseudoalteromonas tunicata to the cellulose-containing surface of the green alga Ulva australis is mediated by a mannose-sensitive haemagglutinin (MSHA-like) pilus. We have identified an MSHA pilus biogenesis gene locus in P. tunicata, termed msh/1/2JKLMNEGFBACDOPQ, which shows significant homology, with respect to its genetic characteristics and organization, to the MSHA pilus biogenesis gene locus of Vibrio cholerae. Electron microscopy studies revealed that P. tunicata wild-type cells express flexible pili peritrichously arranged on the cell surface. A P. tunicata mutant (SM5) with a transposon insertion in the mshJ region displayed a non-piliated phenotype. Using SM5, it has been demonstrated that the MSHA pilus promotes attachment of P. tunicata wild-type cells in polystyrene microtitre plates, as well as to microcrystalline cellulose and to the living surface of U. australis. P. tunicata also demonstrated increased pilus production in response to cellulose and its monomer constituent cellobiose. The MSHA pilus thus functions as a determinant of attachment in P. tunicata, and it is proposed that an understanding of surface sensing mechanisms displayed by P. tunicata will provide insight into specific ecological interactions that occur between this bacterium and higher marine organisms.