A SIMPLE AND SENSITIVE HISTOCHEMICAL METHOD FOR CALCIUM
A SIMPLE AND SENSITIVE HISTOCHEMICAL METHOD FOR CALCIUM
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DOI：
10.3181/00379727-80-19661
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发表时间：
1952-01-01
影响因子：
--
通讯作者：
DAHL, LK
中科院分区：
文献类型：
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作者：
DAHL, LK
本文介绍了Ca的组织化学定义技术。将厚度不超过2-3 mm的薄组织样本在4次更换的95%乙醇中固定36小时，直接包埋在石蜡中，并在没有粘合剂的情况下固定在载玻片上。没有暴露于dist。在这个阶段，水是允许的。然后将封固的切片通过2个二甲苯、2个无水和2个95%乙醇，然后直接放入1%茜素红S的0.1%NH4OH溶液中，最终pH 6.3-6.5，持续2 min。通过用蒸馏水（pH 5.8）温和冲洗除去过量染色剂，然后用2个95%和2个无水乙醇脱水，在2个二甲苯中澄清，并封固在雪松油中。钙沉积物显示为强染色的红橙色区域。当以含铁血黄素存在时，Mg和Fe不会被染色。该方法对表面密度不超过0.0004 μ g/ml的钙敏感。mm2。amts的估计。本组织中的Ca的本技术相关的结果与微量化学定量估计。
A technic is descr. for the histochemical definition of Ca. Thin tissue specimens not exceeding 2-3 mm. in thickness are fixed in 4 changes of 95% ethyl alcohol for 36 hrs., directly embedded into paraffin, and mounted on slides without adhesive. No exposure to dist. water is permitted during this stage. Mounted sections are then taken through 2 xylols, 2 absolute, and 2 95% ethyl alcohols and thence directly into 1% alizarin red S in 0.1% NH4OH, final pH 6.3-6.5, for 2 min. Excess stain is removed by gentle irrigation with distilled H2O (pH 5.8) then dehydrated with 2 95% and 2 absolute alcohols, clarified in 2 xylols and mounted in cedarwood oil. Ca deposits are shown as intensely-staining reddish-orange areas. Mg, and Fe when present as hemosiderin, will not be stained. The method is sensitive to Ca at a surface density of not more than 0.0004 ug./mm2. Estimates of the amts. of Ca present in tissues by this technic correlated well with the results obtained by microchemical quantitative estimates.