Evaluation of sulfobetaine-type polymer resin as an SPE adsorbent in the analysis of trace tetracycline antibiotics in honey.
Evaluation of sulfobetaine-type polymer resin as an SPE adsorbent in the analysis of trace tetracycline antibiotics in honey.
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DOI：
10.1002/jssc.200900520
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发表时间：
2009-11
影响因子：
3.1
通讯作者：
Tomoyasu Tsukamoto;Mari Yasuma;A. Yamamoto;K. Hirayama;Takafumi Kihou;S. Kodama;Y. Inoue
中科院分区：
文献类型：
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作者：
Tomoyasu Tsukamoto;Mari Yasuma;A. Yamamoto;K. Hirayama;Takafumi Kihou;S. Kodama;Y. Inoue
建立了磺基甜菜碱聚合物树脂固相萃取-高效液相色谱-串联质谱法测定蜂蜜中四环素类抗生素残留量的新方法。合成了磺基甜菜碱树脂，并将其填充到一个螺旋型管中，作为选择性吸附TC的固相萃取柱。当上样溶剂为95%v/v乙腈溶液时，TC被定量吸附在磺基甜菜碱柱上，并且吸附的TC不被乙腈水溶液洗脱。使用TFA水溶液洗脱吸附的TC。该方法已应用于蜂蜜样品中TC的测定。蜂蜜样品中TC的回收率为70 - 80%。回收率的降低可能来自于TC在乙腈中的低溶解度。与其他固相萃取树脂相比，该树脂具有上级的选择性，且预处理简单。
A new sulfobetaine polymer resin SPE method combined with HPLC-MS/MS for the determination of tetracycline (TC) antibiotics residues from honey samples is presented. The sulfobetaine resin was synthesized and was packed into a syringe-type tube, which served as the SPE cartridge for selective adsorption of TCs. TCs were quantitatively adsorbed on the sulfobetaine cartridge, when the loading solvent was 95% v/v acetonitrile solution, and TCs adsorbed were not eluted by aqueous acetonitrile washing solution. TFA aqueous solution was used for eluting the adsorbed TCs. The proposed SPE method has been applied to the determination of TCs in honey samples. The recoveries of TCs spiked in honey samples ranged from 70 to 80%. Reduction of the recoveries might be derived from low solubility of TCs in acetonitrile. Compared with other SPE resins, this resin was superior in terms of selectivity with simple pretreatment.