Determination of proline in wine using flow injection analysis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection
Determination of proline in wine using flow injection analysis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection
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DOI：
10.1016/j.talanta.2004.03.065
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发表时间：
2004-11-15
期刊：
影响因子：
6.1
通讯作者：
Lewis, SW
中科院分区：
文献类型：
--
作者：
Costin, JW;Barnett, NW;Lewis, SW
描述了使用三(2,2'-联吡啶)钌(II)化学发光检测测定红葡萄酒和白葡萄酒中脯氨酸的流动注射方法。在 pH 10 下实现了脯氨酸的选择性条件，而其他氨基酸和葡萄酒成分没有干扰。对于标准品 (4 x 10(-6) M) 的 5 次重复，该方法的精密度小于 1.00% (R.S.D.)，检测限为 1 x 10(-8) M。使用开发的方法测定白葡萄酒和起泡酒中脯氨酸的水平与使用 HPLC-FMOC 氨基酸分析获得的水平相当。红葡萄酒需要用 SPE 去除酚类物质，以尽量减少 Ru(bipy)(3)(3+) 的消耗及其对准确性的相关影响。 (C) 2004 Elsevier B.V. 保留所有权利。
Flow injection methodology is described for the determination of proline in red and white wines using tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection. Selective conditions were achieved for proline at pH 10, while other amino acids and wine components did not interfere. The precision of the method was less than 1.00% (R.S.D.) for five replicates of a standard (4 x 10(-6) M) and the detection limit was 1 x 10(-8) M. The level of proline in white and sparkling wines using the developed methodology was equivalent to those achieved using HPLC-FMOC amino acid analysis. SPE removal of phenolic material was required for red wines to minimize Ru(bipy)(3)(3+) consumption and its associated effect on accuracy. (C) 2004 Elsevier B.V. All rights reserved.