Neck and capsid architecture of the robust Agrobacterium phage Milano.
Neck and capsid architecture of the robust Agrobacterium phage Milano.
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DOI：
10.1038/s42003-023-05292-1
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发表时间：
2023-09-08
影响因子：
5.9
通讯作者：
Egelman, Edward H.
中科院分区：
文献类型：
--
作者：
Sonani, Ravi R.;Esteves, Nathaniel C.;Horton, Abigail A.;Kelly, Rebecca J.;Sebastian, Amanda L.;Wang, Fengbin;Kreutzberger, Mark A. B.;Leiman, Petr G.;Scharf, Birgit E.;Egelman, Edward H.
Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the “neck” region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano. Cryo-EM structures of the neck, neck-capsid and neck-tail junctions, and the capsid of the phage Milano reveal a unique collar structure and an extensive network of disulfide bonds providing structural stability to the phage.
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影响因子：
64.8
作者：
Ge P;Scholl D;Prokhorov NS;Avaylon J;Shneider MM;Browning C;Buth SA;Plattner M;Chakraborty U;Ding K;Leiman PG;Miller JF;Zhou ZH
通讯作者：
Zhou ZH
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
--
作者：
Black, Lindsay W.;Rao, Venigalla B.
通讯作者：
Rao, Venigalla B.
影响因子：
5.6
作者：
Fokine, Andrei;Zhang, Zhihong;Kanamaru, Shuji;Bowman, Valorie D.;Aksyuk, Anastasia A.;Arisaka, Fumio;Rao, Venigalla B.;Rossmann, Michael G.
通讯作者：
Rossmann, Michael G.
DOI：
10.1107/s2059798318006551
发表时间：
2018-06-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Poon BK;Read RJ;Sobolev OV;Terwilliger TC;Urzhumtsev A;Adams PD
通讯作者：
Adams PD