Electrochemical biosensor microarray functionalized by means of biomolecule friendly photolithography
Electrochemical biosensor microarray functionalized by means of biomolecule friendly photolithography
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DOI：
10.1016/j.bios.2010.02.012
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发表时间：
2010-05-15
影响因子：
12.6
通讯作者：
Katakis, Ioanis
中科院分区：
文献类型：
--
作者：
Mir, Monica;Kumar Dondapati, Srujan;Katakis, Ioanis
微制造允许在微系统和小体积诊断装置中并入密集电极阵列。然而，具有不同生物分子的任意形状电极的特定功能化仍然是一个具有挑战性的问题。在目前的工作中，制造紧密间隔的微电极（20 μ m的传感器直径和20 μ m间隔的叉指电极阵列），可以选择性地修改，以创建多分析物传感器阵列的问题是通过采用生物分子友好的固定程序顺序固定不同的生物分子到同一阵列的分离的电极。这一概念通过选择性检测用于乳腺癌基因突变检测的寡核苷酸、用特异性抗体检测的激素T4以及用固定在两种分析物阵列中的特异性酶检测的肌氨酸和葡萄糖来证明，以确保该方法与生物传感器中使用的所有类型的生物识别分子兼容。由于这些传感器的低成本、高灵敏度和易于实现，在该阵列中使用了电化学技术。虽然该阵列仅由两组电极组成，但结果表明所提出的方法是通用的，并且可以用于以更高的分辨率图案化电化学多分析物生物传感器。(C)2010 Elsevier BV保留所有权利。
Microfabrication permits the incorporation of dense electrode arrays in microsystems and small volume diagnostic devices. However, the specific functionalization of arbitrary shape electrodes with different biomolecules remains a challenging issue. In the present work, the problem of fabricating closely spaced microelectrodes (20 mu m sensor diameter and 20 mu m-spaced interdigitated electrodes array) that can be modified selectively in order to create multi-analyte sensor arrays is addressed by employing a biomolecule friendly photolithographic procedure for the sequential immobilization of different biomolecules onto separated electrodes of the same array. The concept was demonstrated with selective detection of oligonucleotides for breast cancer gene mutation detection, the hormone T4 detected with specific antibodies and sarcosine and glucose detected with specific enzymes immobilized in two-analyte arrays in order to assure that the method is compatible with all the types of biorecognition molecules used in biosensors. Electrochemical techniques were used in this array, because of the low cost, high sensitivity and easy miniaturisation of these transducers. Although the array was composed of only two sets of electrodes, the results demonstrate that the method proposed is generic and could be used for patterning of electrochemical multi-analyte biosensors at even higher resolution. (C) 2010 Elsevier B.V. All rights reserved.