Differential expression of viral and human interleukin-10 (IL-10) by primary B cell tumors and B cell lines.
Differential expression of viral and human interleukin-10 (IL-10) by primary B cell tumors and B cell lines.
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原代 B 细胞肿瘤和 B 细胞系病毒和人白细胞介素 10 (IL-10) 的差异表达。
DOI：
10.1006/viro.1994.1236
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发表时间：
1994
期刊：
影响因子：
3.7
通讯作者：
Rooney,CM
中科院分区：
文献类型：
--
作者：
Stewart,JP;Behm,FG;Arrand,JR;Rooney,CM
人和病毒白细胞介素-10（IL-10）对人B细胞具有生长因子活性，并可能在B细胞恶性肿瘤中作为自分泌生长因子。为了研究这种可能性，我们测量了EBV阳性和阴性B谱系肿瘤和肿瘤细胞系中的病毒（v）和人（h）IL-10表达。以前的研究表明IL-10在细胞系中表达，现在我们描述了IL-10在原发性伯基特淋巴瘤（BL）肿瘤活检和确定表型的BL系中的表达模式。vIL-10在EBV感染的裂解（生产）阶段表达，但在病毒潜伏期不表达。虽然hIL-10在大多数B细胞系中表达，但在两个BL活检标本中不表达。hIL-10的表达与EBV的存在无关，但与B细胞系的分化状态有关。因此，vIL-10可增强病毒复制部位感染的B细胞的持久性，虽然hIL-10可能是某些BL和EBV转化的B细胞在体内和体外生长的一个因素，但它不是绝对必需的。
Human and viral interleukin-10 (IL-10) possess growth factor activity for human B cells and may act as autocrine growth factors in B cell malignancies. To study this possibility we have measured viral (v) and human (h) IL-10 expression in EBV-positive and negative B lineage tumors and tumor cell lines. Previous studies demonstrated IL-10 expression in cell lines and now we describe the pattern of IL-10 expression in primary Burkitt's lymphoma (BL) tumor biopsies and in BL lines of defined phenotypes. vIL-10 was expressed during the lytic (productive) phase of EBV infection, but not during virus latency. Although hIL-10 was expressed in the majority of B cell lines, it was not expressed in two BL biopsy specimens. Expression of hIL-10 did not correlate with the presence of EBV, but was associated with the differentiation state of the B cell line. Thus, vIL-10 may enhance the persistence of B cells infected at sites of virus replication, and while hIL-10 may be a factor in the growth bothin vivoandin vitroof some BLs and EBV-transformed B cells, it is not an absolute requirement.