Electrospun collagen/poly(L-lactic acid-co-epsilon-caprolactone) hybrid nanofibrous membranes combining with sandwich construction model for cartilage tissue engineering.
Electrospun collagen/poly(L-lactic acid-co-epsilon-caprolactone) hybrid nanofibrous membranes combining with sandwich construction model for cartilage tissue engineering.
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DOI：
10.1166/jnn.2013.7436
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发表时间：
2013-06
影响因子：
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通讯作者：
Xiaomin He;Wei Fu;B. Feng;Hao Wang;Zhenling Liu;Meng Yin;Wei Wang;Jinghao Zheng
中科院分区：
文献类型：
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作者：
Xiaomin He;Wei Fu;B. Feng;Hao Wang;Zhenling Liu;Meng Yin;Wei Wang;Jinghao Zheng
静电纺丝作为一种新型的软骨组织工程支架制造方法，近年来受到了广泛的关注。在本研究中，我们利用静电纺丝技术制备了一种可生物降解的胶原-聚l -乳酸-共ε -己内酯复合纳米纤维膜（PLCL, 75:25），用于软骨组织工程。通过扫描电镜和显微镜分析胶原/PLCL膜的结构和细胞亲和力。将构建的夹层细胞支架体外培养1周后，分别皮下植入裸鼠4、8、12周。术后各时间点进行大体观察、组织学和免疫组织学评价、糖胺聚糖（GAG）分析和杨氏模量测定。电纺丝胶原/PLCL纳米纤维膜能模拟天然ECM，具有良好的细胞亲和性。植入4周、8周和12周后，所有细胞支架均呈现软骨样形态，外观白色、光滑、有光泽。随着植入时间的延长，含软骨基质的GAG丰度明显增加。此外，即使在植入后12周，在构建体中也观察到软骨分布均匀，几乎没有空区。植入12周后，工程软骨的力学性能可达到天然兔耳软骨的83%。这些结果表明，夹心结构的胶原/PLCL纳米纤维膜可以作为软骨组织工程的新途径。
Electrospinning has recently received much attention, showing great potential as a novel scaffold fabrication method for cartilage tissue engineering. In this study, we developed a biodegradable hybrid nanofibrous membrane of collagen and poly(L-lactic acid-co-epsilon-caprolactone) (PLCL, 75:25) by electrospinning for cartilage tissue engineering. The structure and cell affinity of collagen/PLCL membranes were analyzed by scanning electron microscopy (SEM) and microscopy. The sandwiched cell-scaffold constructs were kept in culture for 1 week in vitro and then implanted subcutaneously into nude mice for 4, 8 and 12 weeks. Gross observation, histological and immunohistological evaluation, glycosaminoglycan (GAG) analysis and Young's modulus measurements were performed at each post-implantation time-point. Electrospun collagen/PLCL nanofibrous membranes could mimic the natural ECM and have good cell affinity. All the cell-scaffold constructs showed cartilage-like morphology with a white, smooth and glistening appearance after 4, 8 and 12 weeks of implantation. The abundance of GAG containing cartilaginous matrix appeared to increase greatly with implantation time. Furthermore, well-distributed cartilage and nearly no empty areas were observed in constructs even at 12 weeks post-implantation. In addition, the mechanical properties of the engineered cartilage after 12 weeks of implantation could reach 83% of that of native rabbit auricular cartilage. These results indicate that collagen/PLCL nanofibrous membranes with the sandwich construction model may serve as a new approach for cartilage tissue engineering.