Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F-2).
Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F-2).
复制标题
致瘤鼠血管内皮细胞系（F-2）的建立和表征。
作者：
Ken;K. Tsujioka;Y. Maruguchi;Kazuhiro Ishii;Yoshiki Miyachi;Kagemasa Kuribayashi;Sadao Imamura
利用BALB/c x C57BL/6 F1-nu/nu裸鼠背部皮肤上发生的紫外光诱导肿瘤，建立了新的小鼠细胞系F-2。将超过1 × 10(5)个F-2细胞注射到裸鼠皮肤中会产生快速发展的血管瘤病变。F-2在不添加任何细胞生长因子的情况下，在含10%胎牛血清的Dulbecco改良Eagle培养基培养条件下，F-2的增殖时间为14 h，在融合时呈“鹅卵石”状。F-2能够在Matrigel上快速分化，从而形成精细的网络结构和小管。小管超微结构观察表明，F-2细胞之间通过中间连接相互连接并排列成空间，但细胞质中未发现Weibel-Palade小体。F-2在37℃时也表现出对荧光标记的乙酰化低密度脂蛋白的积极摄取，而在4℃时则没有，并且与凝集素Griffonia simplicifolia I凝集素有明显的结合。成纤维细胞生长因子的加入对F-2细胞的生长没有促进作用。这些发现强烈提示F-2是一种具有致瘤性和血管内皮细胞特性的转化细胞系，可能在血管肿瘤生物学的研究中具有重要意义。
A new murine cell line, designated F-2, was established from an ultraviolet light-induced tumor which developed on the back skin of a BALB/c x C57BL/6 F1-nu/nu nude mouse. More than 1 x 10(5) F-2 cells injected into nude mouse skin produced rapidly developing hemangiomatous lesions. F-2 had a 14-h doubling time under 10% fetal calf serum-containing Dulbecco's modified Eagle's medium culture conditions without any cell growth factor supplementation, and it showed "cobblestone" appearance at confluency. F-2 was able to rapidly differentiate on Matrigel with resultant fine network structure and tubule formation. Ultrastructural observations of the tubule demonstrated that the F-2 cells were connected to each other by intermediate junctions and arranged to form spaces, but that no Weibel-Palade bodies were found in the cytoplasm. F-2 also showed the active uptake of fluorescent-labeled acetylated low-density lipoprotein at 37 degrees C but not at 4 degrees C, and it showed prominent binding to the lectin, Griffonia simplicifolia I agglutinin. The addition of fibroblast growth factor did not facilitate the growth of F-2 cells. These findings strongly suggest that F-2 is a transformed cell line with tumorigenicity and vascular endothelial cell properties, and it may be useful in the study of vascular tumor biology.