Non-invasive real-time imaging of atherosclerosis in mice using ultrasound biomicroscopy
Non-invasive real-time imaging of atherosclerosis in mice using ultrasound biomicroscopy
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DOI：
10.1016/j.atherosclerosis.2006.03.035
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发表时间：
2007-02-01
期刊：
影响因子：
5.3
通讯作者：
Fritsche-Danielson, Regina
中科院分区：
文献类型：
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作者：
Gan, Li-ming;Gronros, Julia;Fritsche-Danielson, Regina
人们越来越需要开发成像技术来研究各种动脉粥样硬化小鼠模型的体内血管形态和功能。使用超声生物显微镜（UBM），我们开发并验证了一种新的成像方案来跟踪动脉粥样硬化小鼠的病变进展。使用 UBM 对具有不同程度动脉粥样硬化的 ApoE 和 LDL 受体双敲除小鼠 (DKO) 和正常对照小鼠进行升主动脉水平成像。在短轴图像中描绘出平均斑块厚度以及斑块面积，并随后与组织学测量结果进行比较。我们发现，该血管部位的斑块面积与面部测量的总斑块负荷密切相关（p < 0.0001）。 UBM 测量的斑块厚度和面积与同一血管区域的组织学测量指数相关（D < 0.0001 分别 p < 0.0001）。此外，在 16 只 32 至 35 周龄的 DKO 小鼠中，UBM 显示升主动脉 IMT 每周显着增加，从 32 周龄试验时的 0.106 +/- 0.108 增加到 35 周龄时的 0.256 +/- 0.345 mm（p = 0.0002）。总之，这种新颖的成像方案为我们提供了一种非侵入性、准确且廉价的方法来跟踪小鼠体内病变进展。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
There are increasing needs to develop imaging techniques to study in vivo vascular morphology and function in various mouse models of atherosclerosis. Using ultrasound biomicroscopy (UBM), we developed and validated a new imaging protocol to follow lesion progression in atherosclerotic mice. ApoE and LDL receptor double knockout mice (DKO) with various degree of atherosclerosis and normal control mice were imaged at the level of the ascending aorta using UBM. Average plaque thickness, as well as plaque area were delineated ill the short-axis images, and were subsequently compared with histological measurements. We showed that plaque area at this vascular site was closely correlated to total plaque burden from en face measurement (p < 0.0001). UBM-measured plaque thickness and area correlated with indices for histology measures from the same vascular region (D < 0.0001 respective p < 0.0001). Furthermore, in 16 DKO mice aged from 32 to 35 weeks, UBM showed significantly weekly increases of IMT in the ascending aorta from 0.106 +/- 0.108 trial at 32 weeks of age to 0.256 +/- 0.345 mm at 35 weeks of age (p = 0.0002). In conclusion, this novel imaging protocol provides us with a non-invasive, accurate and inexpensive way to follow lesion progression in mice in vivo. (c) 2006 Elsevier Ireland Ltd. All rights reserved.