Developmental Validation of the Huaxia Platinum System and application in 3 main ethnic groups of China.
Developmental Validation of the Huaxia Platinum System and application in 3 main ethnic groups of China.
复制标题
华夏白金系统的开发验证及在中国三大民族的应用
DOI：
10.1038/srep31075
复制
发表时间：
2016-08-08
影响因子：
4.6
通讯作者：
Hou Y
中科院分区：
文献类型：
--
作者：
Wang Z;Zhou D;Jia Z;Li L;Wu W;Li C;Hou Y
STRs分布在整个基因组中，突变率较高，在法医、人类学和群体遗传学等遗传学研究中具有很大的应用价值。STR分析现在已应用于法医调查中人类鉴定的各个方面。本工作描述了一种新的通用检测方法——华夏白金系统的发展验证，该系统在一个单一的PCR系统中扩增CODIS核心位点和中国国家数据库中的所有标记。发展验证表明，这种新方法准确、灵敏、可重复性强。华夏白金系统与其他STR系统之间未发现不一致的呼叫。即使用250 pg的人类DNA也可以获得完整的基因型。CMP分别为2.3094 × 10−27、4.3791 × 10−28和6.9118 × 10−27，CPE分别为0.99999999939059、0.99999999989653和0.99999999976386。上述结果表明，该系统具有多态性和信息性，可为国家DNA数据库的建立提供有效的工具，促进国际间的数据共享。
STRs, scattered throughout the genome with higher mutation rate, are attractive to genetic application like forensic, anthropological and population genetics studies. STR profiling has now been applied in various aspects of human identification in forensic investigations. This work described the developmental validation of a novel and universal assay, the Huaxia Platinum System, which amplifies all markers in the expanded CODIS core loci and the Chinese National Database in one single PCR system. Developmental validation demonstrated that this novel assay is accurate, sensitive, reproducible and robust. No discordant calls were observed between the Huaxia Platinum System and other STR systems. Full genotypes could be achieved even with 250 pg of human DNA. Additionally, 402 unrelated individuals from 3 main ethnic groups of China (Han, Uygur and Tibetan) were genotyped to investigate the effectiveness of this novel assay. The CMP were 2.3094 × 10−27, 4.3791 × 10−28and 6.9118 × 10−27, respectively and the CPE were 0.99999999939059, 0.99999999989653 and 0.99999999976386, respectively. Aforementioned results suggested that the Huaxia Platinum System is polymorphic and informative, which provides efficient tool for national DNA database and facilitate international data sharing.