Blood analysis by Raman spectroscopy
Blood analysis by Raman spectroscopy
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DOI：
10.1364/ol.27.002004
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发表时间：
2002-11-15
期刊：
影响因子：
3.6
通讯作者：
Horowitz, GL
中科院分区：
文献类型：
--
作者：
Enejder, AMK;Koo, TW;Horowitz, GL
使用近红外拉曼光谱法，以临床准确度同时测量全血中多种分析物的浓度，而不进行样品处理。用采用非成像光学器件的仪器获取光谱，该仪器使用光散射吸收血细胞对光散射吸收血细胞对光在混浊介质中的拉曼光的激发和发射的影响的蒙特卡罗模拟来设计，拉曼光谱是从31个个体抽取的全血中收集的。通过偏最小二乘（PLS）分析，对葡萄糖、尿素、总蛋白、白蛋白、甘油三酯、红细胞压积和血红蛋白进行定量预测，具有临床相关精度（r（2）值>0.93）。PLS校准光谱的特征与相应分析物光谱的特征的相似性说明预测是基于由拉曼光携带的分子信息。这证明了利用拉曼光谱定量测量高度光散射和吸收介质中的生物分子含量的可行性。(C)2002年美国光学学会。
Concentrations of multiple analytes were simultaneously measured in whole blood with clinical accuracy, without sample processing, using near-infrared Raman spectroscopy, Spectra were acquired with an instrument employing nonimaging optics, designed using Monte Carlo simulations of the influence of light-scattering-absorbing blood cells on the excitation and emission of Raman light in turbid medium, Raman spectra were collected from whole blood drawn from 31 individuals. Quantitative predictions of glucose, urea, total protein, albumin, triglycerides, hematocrit, and hemoglobin were made by moans of partial least-squares (PLS) analysis with clinically relevant precision (r(2) values >0.93). The similarity of the features of the PLS calibration spectra to those of the respective analyte spectra illustrates that the predictions are based on molecular information carried by the Raman light. This demonstrates the feasibility of using Raman spectroscopy for quantitative measurements of biomolecular contents in highly light-scattering and absorbing media. (C) 2002 Optical Society of America.