REQUIREMENTS FOR THE FORMATION OF PLASMID-TRANSDUCING PARTICLES OF BACILLUS-SUBTILIS BACTERIOPHAGE SPP1
REQUIREMENTS FOR THE FORMATION OF PLASMID-TRANSDUCING PARTICLES OF BACILLUS-SUBTILIS BACTERIOPHAGE SPP1
复制标题
DOI：
10.1002/j.1460-2075.1986.tb04706.x
复制
发表时间：
1986-12-01
期刊：
影响因子：
11.4
通讯作者：
TRAUTNER, TA
中科院分区：
文献类型：
--
作者：
ALONSO, JC;LUDER, G;TRAUTNER, TA
我们之前提出，质粒转导 SPP1 颗粒中串联质粒 DNA 的产生是噬菌体引导的质粒 DNA 滚环型复制的结果。当噬菌体和质粒 DNA 之间提供 DNA/DNA 同源性（促进转导）时，这种 DNA 的产生会大大增强。在这里，我们提供的证据表明，在不允许质粒复制的条件下，噬菌体感染后可以进行连环质粒 DNA 的合成。我们还提出，质粒和噬菌体之间天然存在的同源性足以解释在缺乏促进同源性的情况下观察到的转导频率。 > 47 bp 的同源性最大限度地促进质粒转导。重组并不是连环质粒 DNA 合成中的重要部分。
We had previously proposed that the production of concatemeric plasmid DNA in plasmid-transducing SPP1 particles is a consequence of phage-directed rolling-circle-type replication of plasmid DNA. The production of such DNA was greatly enhanced when DNA/DNA homology was provided between phage and plasmid DNAs (facilitation of transduction). Here we present evidence that synthesis of concatemeric plasmid DNA can proceed after phage infection under conditions non-permissive for plasmid replication. We also proposed that the naturally occurring homology between plasmid and phage is sufficient to account for the frequency of transduction observed in the absence of facilitating homology. Homology of > 47 bp gives the maximal facilitation of plasmid transduction. Recombination is not an essential part in the synthesis of concatemeric plasmid DNA.