Preferential association of syntaxin 8 with the early endosome.
Preferential association of syntaxin 8 with the early endosome.
复制标题
突触融合蛋白 8 与早期内体优先结合。
作者：
V. Subramaniam;E. Loh;H. Horstmann;A. Habermann;Y. Xu;J. Coe;G. Griffiths;W. Hong
突触融合蛋白家族的成员在囊泡对接和多种运输事件的融合中起着重要作用。我们已经分子特征的syntaxin 8，syntaxin家族的新成员。克隆的大鼠cDNA的核苷酸序列预测236个残基的多肽与羧基端18个残基的疏水结构域，可以作为膜锚。作为突触融合蛋白的特征，突触融合蛋白8还含有具有形成卷曲螺旋结构的潜力的区域。在已知的突触融合蛋白中，突触融合蛋白8与突触融合蛋白6最同源，突触融合蛋白6主要与高尔基体网络（trans-Golgi network，TGN）相关。通过北方印迹检测，在所有大鼠组织中检测到突触融合蛋白8转录物。针对重组突触融合蛋白8的抗体识别一种27 kDa的蛋白质，该蛋白质富集在含有高尔基体和内体/溶酶体隔室的膜部分中。膜提取物中的突触融合蛋白8可以以依赖于可溶性N-乙基马来酰亚胺敏感因子（NSF）和可溶性NSF附着蛋白（（α）-SNAP）的方式掺入20S蛋白复合物中，表明突触融合蛋白8确实是SNAP受体（SNARE）。间接免疫荧光显微镜显示，syntaxin 8的大部分是本地化的Rab5标记的早期内体。这通过免疫金标记实验证实，该实验显示突触融合蛋白8在早期核内体中的富集及其与Rab5的共标记。
Members of the syntaxin family play a fundamental role in vesicle docking and fusion of diverse transport events. We have molecularly characterized syntaxin 8, a novel member of the syntaxin family. The nucleotide sequence of cloned rat cDNA predicts a polypeptide of 236 residues with a carboxyl-terminal 18-residue hydrophobic domain that may function as a membrane anchor. Characteristic of syntaxins, syntaxin 8 also contain regions that have the potential to form coiled-coil structures. Among the known syntaxins, syntaxin 8 is most homologous to syntaxin 6 which is predominantly associated with the trans-Golgi network (TGN). The syntaxin 8 transcript is detected in all rat tissues examined by northern blot. Antibodies against recombinant syntaxin 8 recognize a 27 kDa protein that is enriched in membrane fractions containing the Golgi apparatus and the endosomal/lysosomal compartments. Syntaxin 8 in membrane extract could be incorporated into a 20S protein complex in a way that is dependent on the soluble N-ethylmaleimide-sensitive factor (NSF) and soluble NSF attachment protein ((alpha)-SNAP), suggesting that syntaxin 8 is indeed a SNAP receptor (SNARE). Indirect immunofluorescence microscopy reveals that the majority of syntaxin 8 is localized to the early endosome marked by Rab5. This is corroborated by immunogold labeling experiments showing enrichment of syntaxin 8 in the early endosome and its co-labeling with Rab5.
影响因子：
3.3
作者：
von Mollard, GF;Stevens, TH
通讯作者：
Stevens, TH
酵母 SEC17 基因产物在功能上与哺乳动物 α-SNAP 蛋白相同。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Griff,IC;Schekman,R;Rothman,JE;Kaiser,CA
通讯作者：
Kaiser,CA