Pelodiscus sinensis embryos infected with Aeromonas hydrophila show higher survival rates than infected Mauremys reevesiis embryos
Pelodiscus sinensis embryos infected with Aeromonas hydrophila show higher survival rates than infected Mauremys reevesiis embryos
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感染嗜水气单胞菌的中华鳖胚胎比感染里氏毛龟胚胎的存活率更高
DOI：
10.1016/j.aquaculture.2019.734660
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发表时间：
2020-03
期刊：
影响因子：
4.5
通讯作者：
Mu Yi
中科院分区：
文献类型：
--
作者：
Zhao Bo;Gao Yang;Yao Qibin;Dai Xiaoxin;Chu Zhangjie;Tao Zhen;Mu Yi
鳖科和瘿蚊科采用不同的适应策略。然而，免疫反应的差异尚未完全了解。本研究以中华盘螺（Pelodiscus sinensis，P. sinensis）胚胎为材料，用气单胞菌（Aeromonasericana，A.结果表明，感染的Maurymysreevesiis（M. reevesiis）胚。感染和未感染M. ReevesiisandP. sinensisembryos进行了探索。总共检测到8，358个差异表达的单基因，其中3，294个和5，064个基因被认为在P中上调和下调。sinensis与M. reevesiis。随机选取6个差异表达基因（DEG）进行qRT-PCR验证，结果与RNA-seq数据一致。分析转录组数据，八个显著上调/下调的免疫基因（碱性转录因子3（BTF 3）;含补丁结构域1（PTCHD 1）; Sep（O-磷酸丝氨酸）tRNA：Sec（硒代半胱氨酸）tRNA合酶（SEPSECS）;含锌指FYVE型16（ZFYVE 16）; EF-手结构域家族成员D2（EFHD 2）;含锌指MYM型4（ZMYM 4）;组蛋白去乙酰化酶9（HDAC 9）、白细胞介素10受体β亚基（IL-10 RB））。通过qRT-PCR检测免疫抑制。在感染后的早期发育阶段，HDAC 9和IL-10 RB表达显著上调，这两种蛋白在P. sinensis，而M. reevesisup首先调节BTF 3和ZFYVE 16以启动B淋巴样细胞的增殖。我们提供了新的见解之间的免疫差异Emydidae和Trionychidae，促进新方法的开发，以治疗细菌感染的海龟。
Emydidae and Trionychidae used different strategies to adapt to the environmental condition. However, the differences in the immune responses are not yet fully understood. In this study,Pelodiscus sinensis(P. sinensis) embryos infected with Aeromonas hydrophila (A. hydrophila) showed significantly higher survival rates than infectedMauremysreevesiis(M. reevesiis) embryos. Transcriptomic profiles of infected and non-infectedM. reevesiisandP. sinensisembryos were explored. In total, 8,358 differentially expressed unigenes were detected, of which 3,294 and 5,064 genes were considered to be up- and down-regulated inP. sinensiscompared to those inM. reevesiis. Six differentially expressed genes (DEGs) were randomly selected for further validation by qRT-PCR, and the results agreed with the RNA-seq data. Analysing the transcriptome data, eight significantly up/down-regulated immune genes (basic transcription factor 3 (BTF3); patched domain containing 1 (PTCHD1); Sep (O-phosphoserine) tRNA: Sec (selenocysteine) tRNA synthase (SEPSECS); zinc finger FYVE-type containing 16 (ZFYVE16); EF-hand domain family member D2 (EFHD2); zinc finger MYM-type containing 4 (ZMYM4); histone deacetylase 9 (HDAC9); interleukin 10 receptor subunit beta (IL-10RB)) were found. Expression patterns of eight selected genes afterA. hydrophilainjection were detected via qRT-PCR. In the early developmental stages of infected embryos, HDAC9 and IL-10RB significantly up-regulated which play a key role in the activation of NK cells and Th cells inP. sinensis, whileM. reevesiisup-regulated BTF3 and ZFYVE16 first to start proliferation of B-lymphoid cells. We provide new insights into the immunity difference between Emydidae and Trionychidae, facilitating the development of new methods to treat bacterial infections in turtles.
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DOI：
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发表时间：
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期刊：
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影响因子：
--
作者：
Jeanine M. Refsnider;F. Janzen
通讯作者：
Jeanine M. Refsnider;F. Janzen
影响因子：
2.8
作者：
Candan, Esra Deniz
通讯作者：
Candan, Esra Deniz
DOI：
10.1002/jez.a.243
发表时间：
2006
期刊：
Journal of experimental zoology. Part A, Comparative experimental biology
影响因子：
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作者：
W. Du;X. Ji
通讯作者：
W. Du;X. Ji
海龟胚胎的行为温度调节
DOI：
10.1073/pnas.1102965108
发表时间：
2011-06-07
影响因子：
11.1
作者：
Du, Wei-Guo;Zhao, Bo;Shine, Richard
通讯作者：
Shine, Richard
影响因子：
4.7
作者：
Li, Xiaoliang;Zhu, Binglin;Fang, Weihuan
通讯作者：
Fang, Weihuan