Overexpression of Pa_1_10620 encoding a mitochondrial Podospora anserina protein with homology to superoxide dismutases and ribosomal proteins leads to lifespan extension
Overexpression of Pa_1_10620 encoding a mitochondrial Podospora anserina protein with homology to superoxide dismutases and ribosomal proteins leads to lifespan extension
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DOI：
10.1007/s00294-014-0446-x
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发表时间：
2015-02
期刊：
影响因子：
2.5
通讯作者：
Carolin Grimm;Lena Böhl;H. Osiewacz
中科院分区：
文献类型：
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作者：
Carolin Grimm;Lena Böhl;H. Osiewacz
在生物系统中，活性氧簇(ROS)是一把“双刃剑”：作为信号分子，它们是正常发育所必需的；作为反应性物质，它们会造成分子损伤和退化、衰老等不良影响。因此，对ROS进行良好的协调控制至关重要。超氧化物歧化酶(SOD)是超氧化物脱毒的活性酶。这些蛋白质的异构体数量因物种而异。在这里，我们报告了由PA_1_10620编码的推定蛋白的特征，该蛋白先前被注释为编码线粒体核糖体蛋白，但也与SODS共享序列结构域。我们报道，该基因在所有年龄的鹅绒委陵菜培养物中转录，并且编码的蛋白定位于线粒体。在PA_1_10620高表达的菌株中，在分离的线粒体中未检测到超氧化物歧化酶活性。然而，该基因的过度表达会导致寿命延长，这表明该蛋白在鹅绒委陵菜中具有促进生存的功能。
In biological systems, reactive oxygen species (ROS) represent ‘double edged swords’: as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Superoxide dismutases (SODs) are enzymes active in the detoxification of superoxide. The number of isoforms of these proteins varies among species. Here we report the characterization of the putative protein encoded byPa_1_10620that has been previously annotated to code for a mitochondrial ribosomal protein but shares also sequence domains with SODs. We report that the gene is transcribed inP.anserinacultures of all ages and that the encoded protein localizes to mitochondria. In strains overexpressingPa_1_10620in a genetic background in whichPaSod3, the mitochondrial MnSOD ofP.anserina, is deleted, no SOD activity could be identified in isolated mitochondria. However, overexpression of the gene leads to lifespan extension suggesting a pro-survival function of the protein inP.anserina.