Biocompatible Interface-Modified Tissue Engineering Chamber Reduces Capsular Contracture and Enlarges Regenerated Adipose Tissue
Biocompatible Interface-Modified Tissue Engineering Chamber Reduces Capsular Contracture and Enlarges Regenerated Adipose Tissue
复制标题
生物相容性界面改良的组织工程室减少包膜挛缩并扩大再生脂肪组织
DOI：
10.1021/acsbiomaterials.8b00930
复制
发表时间：
2019
影响因子：
--
通讯作者：
Lu Feng
中科院分区：
文献类型：
--
作者：
Qin Zijin;Chang Qiang;Lei Chen;He Yunfan;Huang Zhiyong;Xing Malcolm;Lu Feng
使用组织工程室（TEC）进行脂肪瓣扩张，通过激活原位脂肪组织再生，为软组织再生提供了一种有前景的候选方案。然而，硅胶腔引起的异物反应（FBR）和包膜挛缩限制了大组织重建。在这里，在宿主组织和硅胶室之间呈现了由聚乙二醇二丙烯酸酯（PEG-da）和甲基丙烯酸明胶（明胶-MA）制成的亲水性和可生物降解的薄膜，以调节局部伤口并防止 FBR 的启动。经过 60 天的研究，PEG-明胶组获得了 6.1 倍的再生脂肪组织，而硅胶组仅获得了 3 倍的组织。组织学染色表明，两组新生脂肪组织的结构与成熟脂肪组织相似。值得注意的是，与 PEG-明胶组相比，在有机硅组中观察到更明显、更致密的纤维胶囊。 CD206 和 TGF-β 表达的免疫组织化学表明，在 PEG-明胶辅助下，M2 巨噬细胞浸润减少，炎症反应轻微。通过 α-SMA 和 I 型胶原染色证明 PEG-明胶组的胶原沉积和肌成纤维细胞活化较少。所有这些都表明，生物相容性膜补充剂可以减弱胶囊的形成和挛缩，从而通过 TEC 技术实现更大的组织再生，这可能会给材料相关的 FBR 和组织再生之间的关系带来新的视角。
Adipose flap expansion using a tissue engineering chamber (TEC) presents a promising candidate for soft tissue regeneration by activating in situ adipose tissue regeneration. However, foreign body reaction (FBR) and capsular contracture caused by a silicone chamber limit large tissue reconstruction. Here, a hydrophilic and biodegradable film made of poly(ethylene glycol) diacrylate (PEG-da) with methacrylated gelatin (gelatin-MA) was presented between the host tissue and silicone chamber to tune the local wound and to prevent initiation of FBR. After a 60 day investigation, 6.1-fold-regenerated fat tissue was obtained from the PEG–gelatin group, whereas only 3-fold tissue was harvested from a silicone group. Histological staining demonstrated that the structure of the neo-formed adipose tissue in both groups was similar to mature adipose tissue. Noticeably, a more distinct and denser fibrous capsule was observed in the silicone group compared to the PEG–gelatin group. Immunohistochemistry of CD206 and TGF-β expression indicated less M2 macrophage infiltration and a minor inflammation reaction with PEG–gelatin assistance. Less collagen deposition and myofibroblast activation in the PEG–gelatin group were demonstrated via α-SMA and type I collagen staining. All these demonstrated that a biocompatible membrane supplement can attenuate capsule formation and contracture leading to a larger tissue regeneration through the TEC technique, which could lead to new perspectives to the relationship between materials-mattered FBR and tissue regeneration.