Promoter hypermethylation of TMS1, BRCA1, ERα and PRB in serum and tumor DNA of invasive ductal breast carcinoma patients
Promoter hypermethylation of TMS1, BRCA1, ERα and PRB in serum and tumor DNA of invasive ductal breast carcinoma patients
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DOI：
10.1016/j.lfs.2007.05.012
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发表时间：
2007-07-04
期刊：
影响因子：
6.1
通讯作者：
Ralhan, Ranju
中科院分区：
文献类型：
--
作者：
Mirza, Sameer;Sharma, Gayatri;Ralhan, Ranju
乳腺癌正在迅速成为女性中的主要癌症，特别是在印度的年轻人群中;这些肿瘤中的很大一部分通常是侵袭性的，ER和/或PR阴性。参与DNA修复和激素调节的基因的启动子甲基化可能部分解释了这种行为。为了验证这一假设，我们测定了乳腺浸润性导管癌和配对血清DNA中肿瘤抑制基因TMS 1、BRCA 1、ER α和PRB的甲基化状态。在研究的50例乳腺癌患者中，36/50（72%）肿瘤和32/50（64%）配对血清显示至少一个这些基因的甲基化，而17/50（34%）肿瘤和12/50（24%）血清显示三个基因的甲基化。甲基化频率范围从TMS 1的24%到ER的63%（x. ER α和PRB甲基化之间存在显著相关性（p
Breast cancer is fast emerging as the leading cancer amongst females, especially in younger age group in India; a large proportion of these tumors are often aggressive and ER and/or PR negative. Promoter methylation of genes involved in DNA repair and hormonal regulation may, in part, account for this behavior. To test this hypothesis methylation status of tumor suppressor genes TMS1, BRCA1, ER alpha and PRB was determined in invasive ductal carcinoma of breast and paired serum DNA. Of the 50 breast cancer patients investigated, 36/50 (72%) tumors and 32/50 (64%) paired sera showed methylation of at least one of these genes, while 17/50 (34%) tumors and 12/50 (24%) sera showed methylation of three genes. Methylation frequencies ranged from 24% for TMS1 to 63% for ER(x. Significant association was observed between ERa and PRB methylation (p