Properties of supercoiled DNA in gel electrophoresis. The V-like dependence of mobility on topological constraint. DNA-matrix interactions.
Properties of supercoiled DNA in gel electrophoresis. The V-like dependence of mobility on topological constraint. DNA-matrix interactions.
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凝胶电泳中超螺旋 DNA 的特性。
DOI：
10.1016/0022-2836(86)90282-2
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发表时间：
1986
影响因子：
5.6
通讯作者：
A. Prunell
中科院分区：
文献类型：
--
作者：
Y. Zivanovic;I. Goulet;A. Prunell
在丙烯酰胺或琼脂糖凝胶中，研究了DNA小环的电泳率随DNA大小(约350-1400个碱基对)、凝胶浓度和核苷酸序列的变化。在适当调节DNA的大小和凝胶浓度的情况下，这种依赖关系在约束O附近的有限区间内呈V形，对于松弛的DNA，在V的顶点获得最小迁移率。对V的DNA大小依赖关系的分析表明，它是凝胶基质对DNA环进行调制致密的结果。松弛时压实度最大，随着超卷的增加，压实度减小。与这种解释一致，人们发现凝胶可以防止结构偏离Bhelix，如Z转变和十字形挤出，后者往往会放松DNA分子，使其更具扩张性。相反，当DNA大小或凝胶浓度相对于彼此足够大时，观察到的是U形而不是V形，这是因为更接近松弛的环的迁移率增加。讨论了这些结果与体内超螺旋DNA的情况的相关性。介绍了V在DNA螺旋扭度测量中的应用。
The dependence of the electrophoretic mobility of small DNA rings on topological constraint was investigated in acrylamide or agarose gels as a function of DNA size (from approximately 350 to 1400 base-pairs), gel concentration and nucleotide sequence. Under appropriate adjustment between the size of the DNA and the gel concentration, this dependence was found to be V-shaped in a limited interval around constraint O, the minimum mobility at the apex of the V being obtained for relaxed DNA. Analysis of the DNA size dependence of the V suggests that it is the result of a modulated compaction of the DNA rings by the gel matrix. Compaction appears to be maximum upon relaxation, and to decrease with increase in supercoiling. Consistent with this interpretation, gels were found to oppose structural departures from theBhelix, such asZtransition and cruciform extrusion, which tend to relax the DNA molecule and make it more expanded. In contrast, when DNA size or gel concentration are large enough relative to one another, U shapes are observed instead of Vs, as a consequence of an increase in the mobility of the rings closer to relaxation. The relevance of these results to the situation of superhelical DNAin vivois discussed. Application of the V to the measurement of the DNA helical twist is mentioned.
影响因子：
5.6
作者：
SHORE, D;BALDWIN, RL
通讯作者：
BALDWIN, RL
影响因子：
5.6
作者：
HOROWITZ, DS;WANG, JC
通讯作者：
WANG, JC