Natural anti-insulin autoantibodies in cats: Enzyme-linked immunosorbent assay for the determination of plasma anti-insulin IgG and its concentrations in domestic cats
Natural anti-insulin autoantibodies in cats: Enzyme-linked immunosorbent assay for the determination of plasma anti-insulin IgG and its concentrations in domestic cats
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DOI：
10.1016/j.rvsc.2013.08.017
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发表时间：
2013-12-01
影响因子：
2.4
通讯作者：
Kitagawa, Hitoshi
中科院分区：
文献类型：
--
作者：
Takashima, Satoshi;Nishii, Naohito;Kitagawa, Hitoshi
在健康猫的血清中发现抗胰岛素免疫球蛋白G （IgG）。为了确定这些抗体的浓度，建立了抗胰岛素IgG的酶联免疫吸附试验（ELISA）。ELISA在67.5 ~ 2160 ng/ml的标准浓度线上保持线性关系。两种不同血浆样品内测定值的方差系数（cv）分别为4.0%和3.7%。两种不同血浆样品的测定间CVs分别为5.1%和6.9%。两种样品的稀释曲线均为直线。84只健康猫均检测到抗胰岛素IgG。血浆抗胰岛素IgG浓度范围为80 ~ 1578 μ g/ml，中位浓度为221 μ g/ml，与血浆总IgG浓度呈正相关（r = 0.383, p < 0.01）。在静脉葡萄糖耐量试验中，血浆抗胰岛素IgG浓度没有改变，即使血浆葡萄糖和胰岛素浓度发生变化。所建立的酶联免疫吸附试验能够测定家猫的血浆抗胰岛素IgG，并证实所有健康猫均具有血浆抗胰岛素IgG。测定不同病理状态猫的血浆中抗胰岛素IgG的浓度可能有助于阐明抗胰岛素IgG的作用。(C) 2013 Elsevier Ltd.版权所有。
Anti-insulin immunoglobulin G (IgG) has been found in the sera of healthy cats. To determine the concentrations of these antibodies, an enzyme-linked immunosorbent assay (ELISA) for anti-insulin IgG was developed. ELISA maintained the linearity of a standard concentration line between 67.5 and 2160 ng/ml. The coefficients of variances (CVs) of intra-assays in two different plasma samples were 4.0% and 3.7%, respectively. The inter-assay CVs in two different plasma samples were 5.1% and 6.9%, respectively. The dilution curves of two samples were rectilinear. Anti-insulin IgG was detected in all 84 of the healthy cats that were tested. Plasma anti-insulin IgG concentrations ranged from 80 to 1578 mu g/ml, with a median concentration of 221 mu g/ml, and this value correlated positively with total plasma IgG concentrations (r = 0.383, p < 0.01). In an intravenous glucose tolerance test, plasma anti-insulin IgG concentrations did not alter, even with changes in plasma glucose and insulin concentrations. The ELISA that was developed was able to determine plasma anti-insulin IgG in domestic cats, and confirmed that all healthy cats had plasma anti-insulin IgG. Determining the plasma concentrations of anti-insulin IgG in cats with various pathological conditions might clarify the role of anti-insulin IgG. (C) 2013 Elsevier Ltd. All rights reserved.