A defective interference-like phenomenon of human hepatitis B virus in chronic carriers.
A defective interference-like phenomenon of human hepatitis B virus in chronic carriers.
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慢性携带者中人类乙型肝炎病毒的缺陷干扰样现象。
DOI：
10.1128/jvi.72.1.578-584.1998
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发表时间：
1998
影响因子：
5.4
通讯作者：
Shih,C
中科院分区：
文献类型：
--
作者：
Yuan,TT;Lin,MH;Chen,DS;Shih,C
自从在流感病毒中首次发现以来，在细菌、植物和动物的许多RNA和DNA病毒中都发现了缺陷干扰(DI)颗粒。然而，这一基本现象还没有在人类自然感染中得到证明。使用一种新的方法，我们在这里提供了第一个实验证据，证明在人类慢性乙肝病毒携带者中存在DI样病毒。对自然发生的核心内部缺失(CID)变异的功能特征揭示了DI颗粒的所有特征。当将等量的野生型和CID变异体DNA共转染人肝癌细胞系Huh7时，最常观察到CID变异体的三到五倍的丰富。在三个慢性携带者中，CID变异体和辅助乙肝病毒之间的病毒种群的波动让人想起其他DI病毒系统中的循环现象。这一发现对慢性病毒性肝炎和其他慢性进行性病毒性疾病具有重要意义。
Defective interfering (DI) particles have been found in many RNA and DNA viruses of bacteria, plants, and animals since their first discovery in influenza virus. However, this fundamental phenomenon has not been demonstrated in human natural infections. Using a new approach, here we provide the first experimental evidence for the existence of DI-like viruses in human chronic carriers of hepatitis B virus (HBV). Functional characterization of naturally occurring core internal deletion (CID) variants of HBV revealed all of the features of DI particles. When equal amounts of wild-type and CID variant DNAs were cotransfected into a human hepatoma cell line, Huh7, a three- to fivefold enrichment of CID variants was most often observed. The fluctuations of the virus populations between CID variants and helper HBV in three chronic carriers are reminiscent of the cycling phenomenon in other DI viral systems. This finding has important implications for chronic viral hepatitis and other chronic progressive viral diseases.