SCATTER FACTOR INDUCES BLOOD-VESSEL FORMATION INVIVO
SCATTER FACTOR INDUCES BLOOD-VESSEL FORMATION INVIVO
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DOI：
10.1073/pnas.90.5.1937
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发表时间：
1993-03-01
影响因子：
11.1
通讯作者：
ROSEN, EM
中科院分区：
文献类型：
--
作者：
GRANT, DS;KLEINMAN, HK;ROSEN, EM
分散因子（也称为肝细胞生长因子）是由基质细胞分泌的刺激细胞运动和增殖的糖蛋白。在体外，分散因子刺激血管内皮细胞迁移、增殖和组织化成毛细血管样管。使用两种不同的体内试验，我们表明，生理量的纯化天然小鼠散射因子和重组人肝细胞生长因子诱导血管生成（形成新的血管）。血管生成活性被特异性抗散射因子抗体阻断。分散因子诱导培养的微血管内皮细胞积累和分泌尿激酶的量显着增加，与血管生成过程中的侵袭性内皮表型的发展相关的酶。我们进一步表明，免疫反应性散射因子存在于银屑病皮肤血管形成的周围部位。这些发现表明，散射因子可能作为一种旁分泌介质在病理血管生成与人类炎症性疾病。
Scatter factor (also known as hepatocyte growth factor) is a glycoprotein secreted by stromal cells that stimulates cell motility and proliferation. In vitro, scatter factor stimulates vascular endothelial cell migration, proliferation, and organization into capillary-like tubes. Using two different in vivo assays, we showed that physiologic quantities of purified native mouse scatter factor and recombinant human hepatocyte growth factor induce angiogenesis (the formation of new blood vessels). The angiogenic activity was blocked by specific anti-scatter factor antibodies. Scatter factor induced cultured microvascular endothelial cells to accumulate and secrete significantly increased quantities of urokinase, an enzyme associated with development of an invasive endothelial phenotype during angiogenesis. We further showed that immunoreactive scatter factor is present surrounding sites of blood vessel formation in psoriatic skin. These findings suggest that scatter factor may act as a paracrine mediator in pathologic angiogenesis associated with human inflammatory disease.