The oxidation of NADH by vanadate plus sugars.
The oxidation of NADH by vanadate plus sugars.
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钒酸盐和糖对 NADH 的氧化作用。
DOI：
10.1016/0304-4165(87)90029-8
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
I. Fridovich
中科院分区：
文献类型：
--
作者：
S. Liochev;I. Fridovich
糖和磷酸糖使钒酸盐能够催化NADH的氧化。超氧化物歧化酶抑制这种氧化。在添加NADH之前，糖与钒酸盐的孵育加速了随后添加的NADH的氧化，并消除了否则注意到的滞后期。糖与钒酸盐孵育也会导致钒酸盐还原为钒酸盐，其外观呈蓝绿色，可能与钒酸盐-钒酸盐复合物有关。似乎糖将钒酸盐还原为钒基，钒基又将O2还原为O2 -，然后钒酸盐加O2 -通过自由基链反应催化NAD(P)H的氧化。NAD(P)H的这种氧化可能解释了钒酸盐的几种生物效应。
Sugars and sugar phosphates enable vanadate to catalyze the oxidation of NADH. Superoxide dismutase inhibits this oxidation. Incubation of sugars with vanadate, prior to addition of NADH, accelerates this oxidation of subsequently added NADH and eliminates the lag phase otherwise noted. Incubation of sugars with vanadate also results in the reduction of vanadate to vanadyl, with appearance of a blue-green color probably associated with a vanadyl-vanadate complex. It appears that sugars reduce vanadate to vanadyl which, in turn, reduces O2to O2−and that vanadate plus O2−then catalyzes the oxidation of NAD(P)H by a free radical chain reaction. Such oxidation of NAD(P)H may account for several of the biological effects of vanadate.