Effect of nitrosative stress on Schizosaccharomyces pombe: inactivation of glutathione reductase by peroxynitrite.
Effect of nitrosative stress on Schizosaccharomyces pombe: inactivation of glutathione reductase by peroxynitrite.
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亚硝化应激对粟酒裂殖酵母的影响：过氧亚硝酸盐灭活谷胱甘肽还原酶。
DOI：
10.1016/j.freeradbiomed.2005.09.029
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发表时间：
2006
影响因子：
7.4
通讯作者：
Sanjay Ghosh
中科院分区：
文献类型：
--
作者：
Rupam Sahoo;T. Dutta;Amlan Das;S. Sinha Ray;R. Sengupta;Sanjay Ghosh
氧化应激已被证明可以改变不同类型细胞中的细胞氧化还原状态。据报道，在氧化损伤和亚硝化应激过程中，一些抗氧化和抗应激反应基因的表达以及各种蛋白质的激活或失活也发生了变化。在本研究中，我们展示了亚硝化胁迫对裂殖酵母细胞氧化还原状态的影响。本文首次报道了S-亚硝基谷胱甘肽(GSNO)还原酶活性及其灭活方法。我们还发现在活体中存在各种活性氮物种时，谷胱甘肽还原酶(GR)和谷胱甘肽过氧化物酶失活。此外，我们还首次在体内使用S.pombe细胞观察了过氧亚硝酸盐对GR的失活，并在体外条件下进行了类似的观察。一条抗单抗3-硝基酪氨酸抗体的免疫反应带证实了GR在体外条件下的修饰。我们还观察了亚硝化胁迫对对亚硝化胁迫更为敏感的ΔPAP1细胞的影响，表明PAP1参与了对亚硝化胁迫的保护作用。最后，将S.pombe细胞暴露在活性氮物种中，揭示了细胞硫醇库在保护亚硝化胁迫中的重要作用。
Oxidative stress has been shown to alter cellular redox status in various cell types. Changes in expressions of several antioxidative and antistress-responsive genes along with activation or inactivation of various proteins were also reported during oxidative insult as well as during nitrosative stress. In the present study, we show the effect of nitrosative stress on cellular redox status of fission yeast Schizosaccharomyces pombe. This is the first report of S-nitrosoglutathione (GSNO) reductase activity in S. pombe and its inactivation by GSNO. We also show the inactivation of glutathione reductase (GR) and glutathione peroxidase in the presence of various reactive nitrogen species in vivo. In addition, we first observe the inactivation of GR by peroxynitrite in vivo using S. pombe cells and also similar observations under in vitro conditions. An immunoreactive band against monoclonal anti-3-nitrotyrosine antibody confirms the modification of GR under in vitro conditions. We also show the effect of nitrosative stress on Δpap1 cells of S. pombe, which are more sensitive to nitrosative stress, indicating the involvement of Pap1 in the protection against nitrosative stress. Finally, exposure of S. pombe cells to reactive nitrogen species reveals an important role of cellular thiol pool in protection against nitrosative stress.
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影响因子：
4.1
作者：
Alvarez, B;Rubbo, H;Radi, R
通讯作者：
Radi, R
一氧化氮、超氧化物和过氧亚硝酸盐：好的、坏的和丑陋的。
DOI：
10.1152/ajpcell.1996.271.5.c1424
发表时间：
1996
期刊：
The American journal of physiology
影响因子：
--
作者：
Beckman,JS;Koppenol,WH
通讯作者：
Koppenol,WH
DOI：
10.1073/pnas.95.24.14100
发表时间：
1998-11-24
影响因子：
11.1
作者：
Hausladen, A;Gow, AJ;Stamler, JS
通讯作者：
Stamler, JS
影响因子：
4.1
作者：
Beckman, JS
通讯作者：
Beckman, JS
裂殖酵母裂殖酵母乙醇脱氢酶基因的一级结构。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Russell,PR;Hall,BD
通讯作者：
Hall,BD