POTATO-VIRUS X-RELATED SINGLE-STRANDED AND DOUBLE-STRANDED RNAS - CHARACTERIZATION AND IDENTIFICATION OF TERMINAL STRUCTURES
POTATO-VIRUS X-RELATED SINGLE-STRANDED AND DOUBLE-STRANDED RNAS - CHARACTERIZATION AND IDENTIFICATION OF TERMINAL STRUCTURES
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DOI：
10.1016/0014-5793(87)80076-5
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发表时间：
1987-04-20
期刊：
影响因子：
3.5
通讯作者：
ATABEKOV, JG
中科院分区：
文献类型：
--
作者：
DOLJA, VV;GRAMA, DP;ATABEKOV, JG
在感染马铃薯X病毒（PVX）的植物中发现了6种3′-共末端的亚基因组大小的含poly（A）的RNA：2种主要RNA（0.9kb-外壳蛋白mRNA和2.1kb）和4种次要RNA（1.4，1.8，3.0和3.6kb）。最短的sgRNA的5′-末端位于外壳蛋白基因起始密码子上游26个核苷酸处（距离PVX基因组RNA的3′-末端poly（A）区812个核苷酸）。已经发现大多数sgRNA的双链类似物。基因组大小的双链RNA（复制形式）显示在一端携带主要长度为150-250 bp的多聚（A）-多聚（U）杂合体，在另一端（负链的3′端）携带未配对的G残基。与此相反（-），双链0.9和2.1 kbp sgRNA的链缺乏未配对的G，并且两端都是C。
Six species of 3′-coterminal poly(A) -containing RNAs of subgenomic (sg) size have been found in plants infected with potato virus X (PVX): two major (0.9 kb — the coat protein mRNA, and 2.1 kb) and four minor (1.4, 1.8, 3.0 and 3.6 kb). The 5′-end of the shortest sgRNA is located 26 nucleotides upstream of the initiating codon of the coat protein gene (812 nucleotides from the 3′-terminal poly(A) tract of the PVX genomic RNA). Double-stranded analogues have been found for most sgRNAs. The genomic-size double-stranded RNA (the replicative form) is shown to carry a poly(A)-poly(U) hybrid of a predominant length of 150–250 bp on one end, and an unpaired G residue on the other (the 3′-end of the negative chain). In contrast to this(—) the chains of double-stranded 0.9 and 2.1 kbp sgRNAs lack the unpaired G and both end in C.