Determination of pterostilbene in rat plasma by a simple HPLC-UV method and its application in pre-clinical pharmacokinetic study
Determination of pterostilbene in rat plasma by a simple HPLC-UV method and its application in pre-clinical pharmacokinetic study
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DOI：
10.1002/bmc.1254
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发表时间：
2009-12-01
影响因子：
1.8
通讯作者：
Ho, Paul C.
中科院分区：
文献类型：
--
作者：
Lin, Hai-Shu;Yue, Bing-De;Ho, Paul C.
开发并验证了一种简单的 HPLC-UV 方法，用于定量大鼠血浆中具有药理活性的植物抗毒素（3,5-二甲氧基-4'-羟基-反式二苯乙烯）。通过测量 320 nm 处的 UV 吸光度进行测定。紫檀芪和内标 3,5,4'-三甲氧基-反式-芪分别在 5.7 和 9.2 分钟时洗脱。校准曲线 (20-2000 ng/mL) 呈线性 (R-2 > 0.997)。检测和定量的下限分别为 6.7 和 20 ng/mL。 RSD 的日内和日间精密度均低于 6%。分析回收率范围为 95.5 +/- 3.7 至 103.2 +/- 0.7%，而绝对回收率范围为 101.9 +/- 1.1 至 104.9 +/- 4.4%。这种简单的 HPLC 方法随后被应用于在 Sprague-Dawley 大鼠中进行的药代动力学研究。紫檀芪的最终消除半衰期和清除率分别为 96.6 +/- 23.7 分钟和 37.0 +/- 2.5 mL/min/kg，而其绝对口服生物利用度为 12.5 +/- 4.7%。紫檀芪似乎比其天然类似物白藜芦醇具有更好的药代动力学特征。版权所有 (C) 2009 John Wiley & Sons, Ltd.
A simple HPLC-UV method was developed and validated for the quantification of pterostilbene (3,5-dimethoxy-4'-hydroxy-trans-stilbene), a pharmacologically active phytoalexin in rat plasma. The assay was carried out by measuring the UV absorbance at 320 nm. Pterostilbene and the internal standard, 3,5,4'-trimethoxy-trans-stilbene eluted at 5.7 and 9.2 min, respectively. The calibration curve (20-2000 ng/mL) was linear (R-2 > 0.997). The lower limits of detection and of quantification were 6.7 and 20 ng/mL, respectively. The intra- and inter-day precisions in terms of RSD were all lower than 6%. The analytical recovery ranged from 95.5 +/- 3.7 to 103.2 +/- 0.7% while the absolute recovery ranged from 101.9 +/- 1.1 to 104.9 +/- 4.4%. This simple HPLC method was subsequently applied in a pharmacokinetic study carried out in Sprague-Dawley rats. The terminal elimination half-life and clearance of pterostilbene were 96.6 +/- 23.7 min and 37.0 +/- 2.5 mL/min/kg, respectively, while its absolute oral bioavailability was 12.5 +/- 4.7%. Pterostilbene appeared to have better pharmacokinetic characteristics than its natural occurring analog, resveratrol. Copyright (C) 2009 John Wiley & Sons, Ltd.