A novel form of pigment-dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach).
A novel form of pigment-dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach).
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潮间带海洋等足类动物 Eurydice pulchra（leach）中枢神经系统中一种新型色素分散激素。
DOI：
10.1002/cne.22533
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Wilcockson DC
中科院分区：
文献类型：
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作者：
Wilcockson DC
色素分散因子（PDF）是众所周知的生物钟输出因子，它驱动许多昆虫的日常活动节奏。然而，其同系物色素分散激素（PDH）在调节甲壳类动物的昼夜节律和/或周期节律中的作用尚不清楚。潮间带等足类甲壳类动物Eurydice pulchraa具有明确的周期（12.4小时）活动节律。在这项研究中，我们表明这与染色质分散的昼夜节律（24小时）周期平行。作为确定PDH在这些节律中的潜在作用的第一步，我们已经确定了PDH在该物种中表达的一种新形式。由于传统的同源克隆不成功，我们采用免疫鉴定和Edman微测序来确定该肽的初级结构。由此，cDNA克隆确定了核苷酸编码序列，从而便于原位杂交和免疫组织化学描述PDH神经元。我们发现它们在形态上类似于那些协调昆虫昼夜活动节奏的细胞。然而，在同时表达潮汐（活动）和昼夜节律（染色质）节律的动物中，通过定量逆转录酶聚合酶链反应（qRT‐PCR）检测，没有证据表明pdhtranscript的表达具有相应的周期性。因此，我们认为PDH在尿激酶的日/潮定时中的任何作用都不是在转录水平上介导的，而是神经血管释放的节律可能在这种协调中起重要作用。[j] .中国生物医学工程学报，2016,31(5):562 - 575。©2010 Wiley‐Liss, Inc。
Pigment‐dispersing factor (PDF) is well known as a circadian clock output factor, which drives daily activity rhythms in many insects. The role of its homologue, pigment‐dispersing hormone (PDH), in the regulation of circadian and/or circatidal rhythmicity in crustaceans is, however, poorly understood. The intertidal isopod crustacean,Eurydice pulchrahas well‐defined circatidal (12.4‐hour) activity rhythms. In this study we show that this runs parallel to a circadian (24‐hour) cycle of chromatophore dispersion. As a first step in determining the potential role of PDH in these rhythms, we have identified a novel form of PDH expressed in this species. Because conventional homology cloning was unsuccessful, we employed immuno‐identification and Edman microsequencing to determine the primary structure of this peptide. From this, cDNA cloning identified the nucleotide encoding sequence and thus facilitated description of PDH neurons by in situ hybridization and immunohistochemistry. We show them to be morphologically similar to those that co‐ordinate circadian activity rhythms in insects. In animals expressing both tidal (activity) and circadian (chromatophore) rhythms, however, there was no evidence for a corresponding periodicity in the expression ofpdhtranscript, as determined by quantitative reverse transcriptase polymerase chain reaction (qRT‐PCR) inEurydiceheads. It is therefore suggested that any role for PDH in daily/tidal timing inEurydiceis not mediated at the transcriptional level, rather rhythms in neurohemal release may be important in such co‐ordination. J. Comp. Neurol. 519:562–575, 2011. © 2010 Wiley‐Liss, Inc.